PRESERVATION OF H2 PRODUCTION ACTIVITY IN NANOPOROUS LATEX COATINGS OF RHODOPSEUDOMONAS PALUSTRIS CGA009 DURING DRY STORAGE AT AMBIENT TEMPERATURES

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To assess the applicability of latex cell coatings as an "off-the-shelf' biocatalyst, the effect of osmoprotectants, temperature, humidity and O{sub 2} on preservation of H{sub 2} production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H{sub 2} production. Beyond 2 weeks of storage, sorbitol- treated coatings lost all H{sub 2} production activity, whereas considerable H{sub 2} production was still detected in sucrose- and trehalose-stabilized coatings. The relative humidity level at which the coatings were stored had a ... continued below

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Milliken, C.; Piskorska, M.; Soule, T.; Gosse, J.; Flickinger, M.; Smith, G. et al. August 27, 2012.

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To assess the applicability of latex cell coatings as an "off-the-shelf' biocatalyst, the effect of osmoprotectants, temperature, humidity and O{sub 2} on preservation of H{sub 2} production in Rhodopseudomonas palustris coatings was evaluated. Immediately following latex coating coalescence (24 h) and for up to 2 weeks of dry storage, rehydrated coatings containing different osmoprotectants displayed similar rates of H{sub 2} production. Beyond 2 weeks of storage, sorbitol- treated coatings lost all H{sub 2} production activity, whereas considerable H{sub 2} production was still detected in sucrose- and trehalose-stabilized coatings. The relative humidity level at which the coatings were stored had a significant impact on the recovery and subsequent rates of H{sub 2} production. After 4 weeks storage under air at 60% humidity, coatings produced only trace amounts of H{sub 2} (0-0.1% headspace accumulation), whereas those stored at <5% humidity retained 27-53% of their H{sub 2} production activity after 8 weeks of storage. When stored in argon at <5% humidity and room temperature, R. palustris coatings retained full H{sub 2} production activity for 3 months, implicating oxidative damage as a key factor limiting coating storage. Overall, the results demonstrate that biocatalytic latex coatings are an attractive cell immobilization platform for preservation of bioactivity in the dry state.

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  • Journal Name: Microbial Biotechnology

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  • Report No.: SRNL-MS-2012-00158
  • Grant Number: DE-AC09-08SR22470
  • Office of Scientific & Technical Information Report Number: 1072700
  • Archival Resource Key: ark:/67531/metadc840202

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  • August 27, 2012

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  • May 19, 2016, 9:45 a.m.

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  • Dec. 12, 2016, 12:45 p.m.

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Milliken, C.; Piskorska, M.; Soule, T.; Gosse, J.; Flickinger, M.; Smith, G. et al. PRESERVATION OF H2 PRODUCTION ACTIVITY IN NANOPOROUS LATEX COATINGS OF RHODOPSEUDOMONAS PALUSTRIS CGA009 DURING DRY STORAGE AT AMBIENT TEMPERATURES, article, August 27, 2012; United States. (digital.library.unt.edu/ark:/67531/metadc840202/: accessed December 9, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.