THE INTERACTION OF THE Eco RI RESTRICTION ENZYME FROM E.coli WITH NUCLEOTIDES

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The Eco R1 restriction enzyme can be shown to be inhibited by nucleotides which correspond to any part of its known site of phosphodiesterase activity. A series of di-, tetra-, and hexa-nucleotide fragments were synthesized and their effect on the activity of the enzyme upon superhelical Co1 E1 DNA studied. The inhibition caused by the individual mononucleotides were also studied. In general all the nucleotide fragments showed some form of interaction with the enzyme system. Tetranucleotides were stronger inhibitors than dinucleotides, which in turn were stronger inhibitors than the mononucleotides. Within each category of inhibitors, those containing the phosphodiester bond ... continued below

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152 p.

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Hollis, Donald F. November 1, 1979.

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The Eco R1 restriction enzyme can be shown to be inhibited by nucleotides which correspond to any part of its known site of phosphodiesterase activity. A series of di-, tetra-, and hexa-nucleotide fragments were synthesized and their effect on the activity of the enzyme upon superhelical Co1 E1 DNA studied. The inhibition caused by the individual mononucleotides were also studied. In general all the nucleotide fragments showed some form of interaction with the enzyme system. Tetranucleotides were stronger inhibitors than dinucleotides, which in turn were stronger inhibitors than the mononucleotides. Within each category of inhibitors, those containing the phosphodiester bond which is acted upon by the enzyme were the strongest inhibitors. Only those fragments which were consistent with the enzymes site of activity showed competitive inhibition kinetics. Nucleotides which do not fit within the site of phosphodiesterase activity show non-competitive inhibition kinetics.

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152 p.

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  • Related Information: Designation of Academic Dissertation: Doctoral; Academic Degree: Ph. D.; Name of Academic Institution: University of California Berkeley

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  • Report No.: LBL-8484
  • Grant Number: DE-AC02-05CH11231
  • DOI: 10.2172/1022106 | External Link
  • Office of Scientific & Technical Information Report Number: 1022106
  • Archival Resource Key: ark:/67531/metadc837452

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • November 1, 1979

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  • May 19, 2016, 3:16 p.m.

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  • July 26, 2016, 5:43 p.m.

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Hollis, Donald F. THE INTERACTION OF THE Eco RI RESTRICTION ENZYME FROM E.coli WITH NUCLEOTIDES, thesis or dissertation, November 1, 1979; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc837452/: accessed June 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.