Laminin Mediates Tissue-specific Gene Expression in Mammary Epithelia

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Tissue-specific gene expression in mammary epithelium is dependent on the extracellular matrix as well as hormones. There is good evidence that the basement membrane provides signals for regulating beta-casein expression, and that integrins are involved in this process. Here, we demonstrate that in the presence of lactogenic hormones, laminin can direct expression of the beta-casein gene. Mouse mammary epithelial cells plated on gels of native laminin or laminin-entactin undergo functional differentiation. On tissue culture plastic, mammary cells respond to soluble basement membrane or purified laminin, but not other extracellular matrix components, by synthesizing beta-casein. In mammary cells transfected with chloramphenicol ... continued below

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591

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Streuli, Charles H; Schmidhauser, Christian; Bailey, Nina; Yurchenco, Peter; Skubitz, Amy P. N.; Roskelley, Calvin et al. April 1, 1995.

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Tissue-specific gene expression in mammary epithelium is dependent on the extracellular matrix as well as hormones. There is good evidence that the basement membrane provides signals for regulating beta-casein expression, and that integrins are involved in this process. Here, we demonstrate that in the presence of lactogenic hormones, laminin can direct expression of the beta-casein gene. Mouse mammary epithelial cells plated on gels of native laminin or laminin-entactin undergo functional differentiation. On tissue culture plastic, mammary cells respond to soluble basement membrane or purified laminin, but not other extracellular matrix components, by synthesizing beta-casein. In mammary cells transfected with chloramphenicol acetyl transferase reporter constructs, laminin activates transcription from the beta-casein promoter through a specific enhancer element. The inductive effect of laminin on casein expression was specifically blocked by the E3 fragment of the carboxy terminal region of the alpha 1 chain of laminin, by antisera raised against the E3 fragment, and by a peptide corresponding to a sequence within this region. Our results demonstrate that laminin can direct tissue-specific gene expression in epithelial cells through its globular domain.

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591

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  • Journal Name: Journal of Cell Biology; Journal Volume: 129; Journal Issue: 3; Related Information: Journal Publication Date: May 1995

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  • Report No.: LBNL-4367E
  • Grant Number: DE-AC02-05CH11231
  • Grant Number: DE-AC-03-76 SF00098, CA 29995 LTF
  • DOI: 10.1083/jcb.129.3.591 | External Link
  • Office of Scientific & Technical Information Report Number: 1009841
  • Archival Resource Key: ark:/67531/metadc837033

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  • April 1, 1995

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  • May 19, 2016, 3:16 p.m.

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  • June 15, 2016, 8:49 p.m.

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Streuli, Charles H; Schmidhauser, Christian; Bailey, Nina; Yurchenco, Peter; Skubitz, Amy P. N.; Roskelley, Calvin et al. Laminin Mediates Tissue-specific Gene Expression in Mammary Epithelia, article, April 1, 1995; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc837033/: accessed September 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.