Microbial Gas Generation Under Expected Waste Isolation Pilot Plant Repository Conditions: Final Report Metadata

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  • Main Title Microbial Gas Generation Under Expected Waste Isolation Pilot Plant Repository Conditions: Final Report


  • Author: Gillow, J. B.
    Creator Type: Personal
  • Author: Francis, A.
    Creator Type: Personal


  • Sponsor: United States. Department of Energy. Office of Science.
    Contributor Type: Organization
    Contributor Info: DOE Office of Science


  • Name: Brookhaven National Laboratory
    Place of Publication: United States


  • Creation: 2011-07-01


  • English


  • Content Description: Gas generation from the microbial degradation of the organic constituents of transuranic (TRU) waste under conditions expected in the Waste Isolation Pilot Plant (WIPP) was investigated. The biodegradation of mixed cellulosic materials and electron-beam irradiated plastic and rubber materials (polyethylene, polyvinylchloride, hypalon, leaded hypalon, and neoprene) was examined. We evaluated the effects of environmental variables such as initial atmosphere (air or nitrogen), water content (humid ({approx}70% relative humidity, RH) and brine inundated), and nutrient amendments (nitogen phosphate, yeast extract, and excess nitrate) on microbial gas generation. Total gas production was determined by pressure measurement and carbon dioxide (CO{sub 2}) and methane (CH{sub 4}) were analyzed by gas chromatography; cellulose degradation products in solution were analyzed by high-performance liquid chromatography. Microbial populations in the samples were determined by direct microscopy and molecular analysis. The results of this work are summarized.


  • Keyword: Cellulose
  • Keyword: Wipp
  • Keyword: Plastics
  • Keyword: Wastes
  • STI Subject Categories: 54 Environmental Sciences
  • Keyword: Rubbers
  • Keyword: Nutrients
  • Keyword: Yeasts
  • Keyword: Humidity
  • Keyword: Microscopy
  • Keyword: Methane
  • STI Subject Categories: 03 Natural Gas
  • Keyword: Biodegradation
  • Keyword: Neoprene
  • Keyword: Gas Chromatography
  • Keyword: High-Performance Liquid Chromatography
  • Keyword: Nitrogen
  • Keyword: Pressure Measurement
  • Keyword: Carbon Dioxide
  • Keyword: Production
  • Keyword: Brines
  • STI Subject Categories: 12 Management Of Radioactive Wastes, And Non-Radioactive Wastes From Nuclear Facilities


  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI


  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Report


  • Text


  • Report No.: BNL--96148-2011-IR
  • Grant Number: DE-AC02-98CH10886
  • Office of Scientific & Technical Information Report Number: 1026774
  • Archival Resource Key: ark:/67531/metadc836055