A molecularly defined duplication set for the X chromosome of Drosophila melanogaster

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We describe a molecularly defined duplication kit for the X chromosome of Drosophila melanogaster. A set of 408 overlapping P[acman] BAC clones was used to create small duplications (average length 88 kb) covering the 22-Mb sequenced portion of the chromosome. The BAC clones were inserted into an attP docking site on chromosome 3L using C31 integrase, allowing direct comparison of different transgenes. The insertions complement 92% of the essential and viable mutations and deletions tested, demonstrating that almost all Drosophila genes are compact and that the current annotations of the genome are reasonably accurate. Moreover, almost all genes are tolerated ... continued below

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Venken, Koen J. T.; Popodi, Ellen; Holtzman, Stacy L.; Schulze, Karen L.; Park, Soo; Carlson, Joseph W. et al. July 22, 2010.

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We describe a molecularly defined duplication kit for the X chromosome of Drosophila melanogaster. A set of 408 overlapping P[acman] BAC clones was used to create small duplications (average length 88 kb) covering the 22-Mb sequenced portion of the chromosome. The BAC clones were inserted into an attP docking site on chromosome 3L using C31 integrase, allowing direct comparison of different transgenes. The insertions complement 92% of the essential and viable mutations and deletions tested, demonstrating that almost all Drosophila genes are compact and that the current annotations of the genome are reasonably accurate. Moreover, almost all genes are tolerated at twice the normal dosage. Finally, we more precisely mapped two regions at which duplications cause diplo-lethality in males. This collection comprises the first molecularly defined duplication set to cover a whole chromosome in a multicellular organism. The work presented removes a long-standing barrier to genetic analysis of the Drosophila X chromosome, will greatly facilitate functional assays of X-linked genes in vivo, and provides a model for functional analyses of entire chromosomes in other species.

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  • Journal Name: Genetics; Journal Volume: 186; Journal Issue: 4

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  • Report No.: LBNL-4329E
  • Grant Number: DE-AC02-05CH11231
  • Grant Number: 1R01 GM080415
  • DOI: 10.1534/genetics.110.121285 | External Link
  • Office of Scientific & Technical Information Report Number: 1008329
  • Archival Resource Key: ark:/67531/metadc835933

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  • July 22, 2010

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  • May 19, 2016, 3:16 p.m.

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  • June 15, 2016, 9 p.m.

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Venken, Koen J. T.; Popodi, Ellen; Holtzman, Stacy L.; Schulze, Karen L.; Park, Soo; Carlson, Joseph W. et al. A molecularly defined duplication set for the X chromosome of Drosophila melanogaster, article, July 22, 2010; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc835933/: accessed September 18, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.