Fluorescence measurements for evaluating the application of multivariate analysis techniques to optically thick environments.

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Laser-induced fluorescence measurements of cuvette-contained laser dye mixtures are made for evaluation of multivariate analysis techniques to optically thick environments. Nine mixtures of Coumarin 500 and Rhodamine 610 are analyzed, as well as the pure dyes. For each sample, the cuvette is positioned on a two-axis translation stage to allow the interrogation at different spatial locations, allowing the examination of both primary (absorption of the laser light) and secondary (absorption of the fluorescence) inner filter effects. In addition to these expected inner filter effects, we find evidence that a portion of the absorbed fluorescence is re-emitted. A total of 688 ... continued below

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24 p.

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Reichardt, Thomas A.; Timlin, Jerilyn Ann; Jones, Howland D. T.; Sickafoose, Shane M. & Schmitt, Randal L. September 1, 2010.

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Laser-induced fluorescence measurements of cuvette-contained laser dye mixtures are made for evaluation of multivariate analysis techniques to optically thick environments. Nine mixtures of Coumarin 500 and Rhodamine 610 are analyzed, as well as the pure dyes. For each sample, the cuvette is positioned on a two-axis translation stage to allow the interrogation at different spatial locations, allowing the examination of both primary (absorption of the laser light) and secondary (absorption of the fluorescence) inner filter effects. In addition to these expected inner filter effects, we find evidence that a portion of the absorbed fluorescence is re-emitted. A total of 688 spectra are acquired for the evaluation of multivariate analysis approaches to account for nonlinear effects.

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24 p.

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  • Report No.: SAND2010-6571
  • Grant Number: AC04-94AL85000
  • DOI: 10.2172/1008131 | External Link
  • Office of Scientific & Technical Information Report Number: 1008131
  • Archival Resource Key: ark:/67531/metadc834660

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  • September 1, 2010

Added to The UNT Digital Library

  • May 19, 2016, 3:16 p.m.

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  • Nov. 28, 2016, 4:39 p.m.

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Reichardt, Thomas A.; Timlin, Jerilyn Ann; Jones, Howland D. T.; Sickafoose, Shane M. & Schmitt, Randal L. Fluorescence measurements for evaluating the application of multivariate analysis techniques to optically thick environments., report, September 1, 2010; United States. (digital.library.unt.edu/ark:/67531/metadc834660/: accessed August 22, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.