Deciphering the details of RNA aminoglycoside interactions: from atomistic models to biotechnological applications

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A detailed study was done of the neomycin-B RNA aptamer for determining its selectivity and binding ability to both neomycin– and kanamycin-class aminoglycosides. A novel method to increase drug concentrations in cells for more efficiently killing is described. To test the method, a bacterial model system was adopted and several small RNA molecules interacting with aminoglycosides were cloned downstream of T7 RNA polymerase promoter in an expression vector. Then, the growth analysis of E. coli expressing aptamers was observed for 12-hour period. Our analysis indicated that aptamers helped to increase the intracellular concentration of aminoglycosides thereby increasing their efficacy.

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Ilgu, Muslum July 23, 2012.

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This thesis or dissertation is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 57 times . More information about this document can be viewed below.

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  • Ames Laboratory
    Publisher Info: Ames Laboratory (AMES), Ames, IA (United States)
    Place of Publication: Ames, Iowa

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A detailed study was done of the neomycin-B RNA aptamer for determining its selectivity and binding ability to both neomycin– and kanamycin-class aminoglycosides. A novel method to increase drug concentrations in cells for more efficiently killing is described. To test the method, a bacterial model system was adopted and several small RNA molecules interacting with aminoglycosides were cloned downstream of T7 RNA polymerase promoter in an expression vector. Then, the growth analysis of E. coli expressing aptamers was observed for 12-hour period. Our analysis indicated that aptamers helped to increase the intracellular concentration of aminoglycosides thereby increasing their efficacy.

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16.8 MB

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  • Report No.: IS-T 3070
  • Grant Number: DE-AC02-07CH11358
  • DOI: 10.2172/1048539 | External Link
  • Office of Scientific & Technical Information Report Number: 1048539
  • Archival Resource Key: ark:/67531/metadc834107

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • July 23, 2012

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  • May 19, 2016, 9:45 a.m.

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  • Aug. 3, 2016, 3:04 p.m.

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Ilgu, Muslum. Deciphering the details of RNA aminoglycoside interactions: from atomistic models to biotechnological applications, thesis or dissertation, July 23, 2012; Ames, Iowa. (digital.library.unt.edu/ark:/67531/metadc834107/: accessed January 16, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.