Comparative Studies on the Carbohydrate Composition of Marine Macroalgae: Final Technical Report Page: 3 of 34
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I. Phaeophyta (brown)
Sargassum filipendula
II. Rhodophyta (red)
Solieria tenera
Hypnea musciformis
III. Chlorophyta (green-)
Enteromorpha intestinalis
all species cited were collected on March 15, 1984 from jetty rocks in the
mouth of the Tampa Bay. Water temperature was 21 C and salinity was 32 ppt.
Samples were taxonomically classified, air dried and provided to the Jackson
State University laboratory courtesy of Dr. Clinton Dawes (Phycologist),
University of South Florida.
Carbohydrate Extraction
Carbohydrates were extracted from algal samples by the method of Mian
and Percival [1] with modifications. One of the major modifications was
homogenization of air dried samples with acetone (10 ml/g) to remove pig-
ments. Homogenization was accomplished utilizing a Brinkman Polytron Homo-
genizer. Homogenized samples were centrifuged and resulting sediments were
washed three times with 200 ml of acetone for 30 minutes while stirring.
Acetone was removed by nitrogen evaporation and subsequently dried in a
vacuum oven at 50 C for 24 hrs. (see fig. 1).
Acetone washed algal samples (25g) were extracted exhaustively with
cold and hot (70 ) 80% aqueous ethanol with constant stirring. The
residual seaweed, recovered by centrifugation, was immersed overnight in
40% formaldehyde. The formaldehyde was decanted off and the air-dried
weed was then sequentially extracted, with constant stirring, with-the
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Mack, R. W. Comparative Studies on the Carbohydrate Composition of Marine Macroalgae: Final Technical Report, report, January 1, 1985; Golden, Colorado. (https://digital.library.unt.edu/ark:/67531/metadc829758/m1/3/: accessed April 20, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.