On-Chip Dielectrophoretic Separation and Concentration of Viable, Non-Viable and Viable but Not Culturable (VBNC) Escherichia coli

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Although bacterial culture remains the gold standard for detection of viable bacteria in environmental specimens, the typical time requirement of twenty-four hours can delay and even jeopardize appropriate public health intervention. In addition, culture is incapable of detecting viable but not culturable (VBNC) species. Conversely, nucleic acid and antibody-based methods greatly decrease time to detection but rarely characterize viability of the bacteria detected. Through selection by membrane permeability, the method described in this work employs positive dielectrophoresis (pDEP) for separation and purification of viable and VBNC species from water and allows concentration of bacteria for downstream applications.

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Packard, M M; Shusteff, M & Alocilja, E C April 12, 2012.

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Although bacterial culture remains the gold standard for detection of viable bacteria in environmental specimens, the typical time requirement of twenty-four hours can delay and even jeopardize appropriate public health intervention. In addition, culture is incapable of detecting viable but not culturable (VBNC) species. Conversely, nucleic acid and antibody-based methods greatly decrease time to detection but rarely characterize viability of the bacteria detected. Through selection by membrane permeability, the method described in this work employs positive dielectrophoresis (pDEP) for separation and purification of viable and VBNC species from water and allows concentration of bacteria for downstream applications.

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PDF-file: 6 pages; size: 0.8 Mbytes

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  • Presented at: TechConnect World 2012 - Nanotech, Microtech, Biotech, Cleantech Joint 2012 Conferences, Santa Clara, CA, United States, Jun 18 - Jun 21, 2012

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  • Report No.: LLNL-CONF-548191
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 1046096
  • Archival Resource Key: ark:/67531/metadc828046

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Office of Scientific & Technical Information Technical Reports

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  • April 12, 2012

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  • May 19, 2016, 9:45 a.m.

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  • Nov. 30, 2016, 2:07 p.m.

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Packard, M M; Shusteff, M & Alocilja, E C. On-Chip Dielectrophoretic Separation and Concentration of Viable, Non-Viable and Viable but Not Culturable (VBNC) Escherichia coli, article, April 12, 2012; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc828046/: accessed August 15, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.