Biological Applications of a Strongly Luminescent Platinum (II) Complex in Reactive Oxygen Species Scavenging and Hypoxia Imaging in Caenorhabditis elegans Metadata

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Title

  • Main Title Biological Applications of a Strongly Luminescent Platinum (II) Complex in Reactive Oxygen Species Scavenging and Hypoxia Imaging in Caenorhabditis elegans

Creator

  • Author: Kinyanjui, Sophia Nduta
    Creator Type: Personal

Contributor

  • Chair: Omary, Mohammad A., 1969-
    Contributor Type: Personal
    Contributor Info: Major Professor
  • Chair: Padilla, Pamela Anne
    Contributor Type: Personal
    Contributor Info: Major Professor
  • Committee Member: Petros, Robby A.
    Contributor Type: Personal
  • Committee Member: Cundari, Thomas R., 1964-
    Contributor Type: Personal

Publisher

  • Name: University of North Texas
    Place of Publication: Denton, Texas
    Additional Info: www.unt.edu

Date

  • Creation: 2015-12

Language

  • English

Description

  • Content Description: Phosphorescent transition metal complexes make up an important group of compounds that continues to attract intense research owing to their intrinsic bioimaging applications that arise from bright emissions, relatively long excited state lifetimes, and large stokes shifts. Now for biomaging assay a model organism is required which must meet certain criteria for practical applications. The organism needs to be small, with a high turn-over of progeny (high fecundity), a short lifecycle, and low maintenance and assay costs. Our model organism C. elegans met all the criteria. The ideal phosphor has low toxicity in the model organism. In this work the strongly phosphorescent platinum (II) pyrophosphito-complex was tested for biological applications as a potential in vivo hypoxia sensor. The suitability of the phosphor was derived from its water solubility, bright phosphorescence at room temperature, and long excited state lifetime (~ 10 µs). The applications branched off to include testing of C. elegans survival when treated with the phosphor, which included lifespan and fecundity assays, toxicity assays including the determination of the LC50, and recovery after paraquat poisoning. Quenching experiments were performed using some well knows oxygen derivatives, and the quenching mechanisms were derived from Stern-Volmer plots. Reaction stoichiometries were derived from Job plots, while percent scavenging (or antioxidant) activities were determined graphically. The high photochemical reactivity of the complex was clearly manifested in these reactions.
  • Physical Description: xvii, 137 pages : illustrations (chiefly color)

Subject

  • Keyword: C. elegans
  • Keyword: phosphorescence
  • Keyword: PtPOP
  • Keyword: ROS scavenging
  • Keyword: hypoxia
  • Library of Congress Subject Headings: Caenorhabditis elegans.
  • Library of Congress Subject Headings: Anoxemia.
  • Library of Congress Subject Headings: Imaging systems in biology.
  • Library of Congress Subject Headings: Luminescence spectroscopy.
  • Library of Congress Subject Headings: Platinum -- Spectra.

Collection

  • Name: UNT Theses and Dissertations
    Code: UNTETD

Institution

  • Name: UNT Libraries
    Code: UNT

Rights

  • Rights Access: public
  • Rights Holder: Kinyanjui, Sophia Nduta
  • Rights License: copyright
  • Rights Statement: Copyright is held by the author, unless otherwise noted. All rights Reserved.

Resource Type

  • Thesis or Dissertation

Format

  • Text

Identifier

  • Archival Resource Key: ark:/67531/metadc822774

Degree

  • Academic Department: Department of Chemistry
  • Degree Discipline: Chemistry - Inorganic Chemistry
  • Degree Level: Doctoral
  • Degree Name: Doctor of Philosophy
  • Degree Grantor: University of North Texas
  • Degree Publication Type: disse

Note