Biological Applications of a Strongly Luminescent Platinum (II) Complex in Reactive Oxygen Species Scavenging and Hypoxia Imaging in Caenorhabditis elegans Metadata
Metadata describes a digital item, providing (if known) such information as creator, publisher, contents, size, relationship to other resources, and more. Metadata may also contain "preservation" components that help us to maintain the integrity of digital files over time.
- Main Title Biological Applications of a Strongly Luminescent Platinum (II) Complex in Reactive Oxygen Species Scavenging and Hypoxia Imaging in Caenorhabditis elegans
Author: Kinyanjui, Sophia NdutaCreator Type: Personal
Chair: Omary, Mohammad A., 1969-Contributor Type: PersonalContributor Info: Major Professor
Chair: Padilla, Pamela AnneContributor Type: PersonalContributor Info: Major Professor
Committee Member: Petros, Robby A.Contributor Type: Personal
Committee Member: Cundari, Thomas R., 1964-Contributor Type: Personal
Name: University of North TexasPlace of Publication: Denton, TexasAdditional Info: www.unt.edu
- Creation: 2015-12
- Content Description: Phosphorescent transition metal complexes make up an important group of compounds that continues to attract intense research owing to their intrinsic bioimaging applications that arise from bright emissions, relatively long excited state lifetimes, and large stokes shifts. Now for biomaging assay a model organism is required which must meet certain criteria for practical applications. The organism needs to be small, with a high turn-over of progeny (high fecundity), a short lifecycle, and low maintenance and assay costs. Our model organism C. elegans met all the criteria. The ideal phosphor has low toxicity in the model organism. In this work the strongly phosphorescent platinum (II) pyrophosphito-complex was tested for biological applications as a potential in vivo hypoxia sensor. The suitability of the phosphor was derived from its water solubility, bright phosphorescence at room temperature, and long excited state lifetime (~ 10 µs). The applications branched off to include testing of C. elegans survival when treated with the phosphor, which included lifespan and fecundity assays, toxicity assays including the determination of the LC50, and recovery after paraquat poisoning. Quenching experiments were performed using some well knows oxygen derivatives, and the quenching mechanisms were derived from Stern-Volmer plots. Reaction stoichiometries were derived from Job plots, while percent scavenging (or antioxidant) activities were determined graphically. The high photochemical reactivity of the complex was clearly manifested in these reactions.
- Physical Description: xvii, 137 pages : illustrations (chiefly color)
- Keyword: C. elegans
- Keyword: phosphorescence
- Keyword: PtPOP
- Keyword: ROS scavenging
- Keyword: hypoxia
- Library of Congress Subject Headings: Caenorhabditis elegans.
- Library of Congress Subject Headings: Anoxemia.
- Library of Congress Subject Headings: Imaging systems in biology.
- Library of Congress Subject Headings: Luminescence spectroscopy.
- Library of Congress Subject Headings: Platinum -- Spectra.
Name: UNT Theses and DissertationsCode: UNTETD
Name: UNT LibrariesCode: UNT
- Rights Access: public
- Rights Holder: Kinyanjui, Sophia Nduta
- Rights License: copyright
- Rights Statement: Copyright is held by the author, unless otherwise noted. All rights Reserved.
- Thesis or Dissertation
- Archival Resource Key: ark:/67531/metadc822774
- Academic Department: Department of Chemistry
- Degree Discipline: Chemistry - Inorganic Chemistry
- Degree Level: Doctoral
- Degree Name: Doctor of Philosophy
- Degree Grantor: University of North Texas
- Degree Publication Type: disse