Distinct constrictive processes, separated in time and space,divide Caulobacter inner and outer membranes

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Cryo-electron microscope tomography (cryoEM) and a fluorescence loss in photobleaching (FLIP) assay were used to characterize progression of the terminal stages of Caulobacter crescentus cell division. Tomographic cryoEM images of the cell division site show separate constrictive processes closing first the inner, and then the outer, membrane in a manner distinctly different from septum-forming bacteria. The smallest observed pre-fission constrictions were 60 nm for both the inner and outer membrane. FLIP experiments had previously shown cytoplasmic compartmentalization, when cytoplasmic proteins can no longer diffuse between the two nascent progeny cell compartments, occurring 18 min before daughter cell separation in a ... continued below

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Judd, Ellen M.; Comolli, Luis R.; Chen, Joseph C.; Downing,Kenneth H.; Moerner, W.E. & McAdams, Harley H. May 1, 2005.

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Cryo-electron microscope tomography (cryoEM) and a fluorescence loss in photobleaching (FLIP) assay were used to characterize progression of the terminal stages of Caulobacter crescentus cell division. Tomographic cryoEM images of the cell division site show separate constrictive processes closing first the inner, and then the outer, membrane in a manner distinctly different from septum-forming bacteria. The smallest observed pre-fission constrictions were 60 nm for both the inner and outer membrane. FLIP experiments had previously shown cytoplasmic compartmentalization, when cytoplasmic proteins can no longer diffuse between the two nascent progeny cell compartments, occurring 18 min before daughter cell separation in a 135 min cell cycle. Here, we used FLIP experiments with membrane-bound and periplasmic fluorescent proteins to show that (1) periplasmic compartmentalization occurs after cytoplasmic compartmentalization, consistent with the cryoEM observations, and (2) inner membrane and periplasmic proteins can diffuse past the FtsZ constriction site, indicating that the cell division machinery does not block membrane diffusion.

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  • Journal Name: Journal of Bacteriology; Journal Volume: 187; Journal Issue: 20; Related Information: Journal Publication Date: 10/2005

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  • Report No.: LBNL--58281
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 862180
  • Archival Resource Key: ark:/67531/metadc794855

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  • May 1, 2005

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  • Dec. 19, 2015, 7:14 p.m.

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  • April 1, 2016, 7:57 p.m.

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Judd, Ellen M.; Comolli, Luis R.; Chen, Joseph C.; Downing,Kenneth H.; Moerner, W.E. & McAdams, Harley H. Distinct constrictive processes, separated in time and space,divide Caulobacter inner and outer membranes, article, May 1, 2005; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc794855/: accessed June 22, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.