Spatially resolved characterization of biogenic manganese oxideproduction within a bacterial biofilm

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Pseudomonas putida strain MnB1, a biofilm forming bacteria, was used as a model for the study of bacterial Mn oxidation in freshwater and soil environments. The oxidation of Mn{sub (aq)}{sup +2} by P. putida was characterized by spatially and temporally resolving the oxidation state of Mn in the presence of a bacterial biofilm using scanning transmission x-ray microscopy (STXM) combined with near edge x-ray absorption fine structure (NEXAFS) spectroscopy at the Mn-L{sub 2,3} absorption edges. Subsamples were collected from growth flasks containing 0.1 mM and 1 mM total Mn at 16, 24, 36 and 48 hours after inoculation. Immediately after ... continued below

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Toner, Brandy; Fakra, Sirine; Villalobos, Mario; Warwick, Tony & Sposito, Garrison October 1, 2004.

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Pseudomonas putida strain MnB1, a biofilm forming bacteria, was used as a model for the study of bacterial Mn oxidation in freshwater and soil environments. The oxidation of Mn{sub (aq)}{sup +2} by P. putida was characterized by spatially and temporally resolving the oxidation state of Mn in the presence of a bacterial biofilm using scanning transmission x-ray microscopy (STXM) combined with near edge x-ray absorption fine structure (NEXAFS) spectroscopy at the Mn-L{sub 2,3} absorption edges. Subsamples were collected from growth flasks containing 0.1 mM and 1 mM total Mn at 16, 24, 36 and 48 hours after inoculation. Immediately after collection, the unprocessed hydrated subsamples were imaged at 40 nm resolution. Manganese NEXAFS spectra were extracted from x-ray energy sequences of STXM images (stacks) and fit with linear combinations of well characterized reference spectra to obtain quantitative relative abundances of Mn(II), Mn(III) and Mn(IV). Careful consideration was given to uncertainty in the normalization of the reference spectra, choice of reference compounds, and chemical changes due to radiation damage. The STXM results confirm that Mn{sub (aq)}{sup +2} was removed from solution by P. putida and was concentrated as Mn(III) and Mn(IV) immediately adjacent to the bacterial cells. The Mn precipitates were completely enveloped by bacterial biofilm material. The distribution of Mn oxidation states was spatially heterogeneous within and between the clusters of bacterial cells. Scanning transmission x-ray microscopy is a promising tool to advance the study of hydrated interfaces between minerals and bacteria, particularly in cases where the structure of bacterial biofilms needs to be maintained.

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  • Journal Name: Applied Environmental Microbiology; Journal Volume: 71; Journal Issue: 3; Related Information: Journal Publication Date: 03/2005

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  • Report No.: LBNL--56948
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 861406
  • Archival Resource Key: ark:/67531/metadc793473

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  • October 1, 2004

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  • Dec. 19, 2015, 7:14 p.m.

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  • April 1, 2016, 8:17 p.m.

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Toner, Brandy; Fakra, Sirine; Villalobos, Mario; Warwick, Tony & Sposito, Garrison. Spatially resolved characterization of biogenic manganese oxideproduction within a bacterial biofilm, article, October 1, 2004; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc793473/: accessed December 13, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.