A biological/chemical process for reduced waste and energy consumption, Caprolactam production: Phase 1, Select microorganisms and demonstrate feasibility. Final report Page: 14 of 49
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caprolactone is metabolized further by a caprolactone hydrolase that hydrolyses
the lactone to an acid-alcohol, 6-hydroxy-hexanoic acid. This product would be
metabolized further to CO2 and H20, while providing carbon and energy to
support the growth of the microorganisms. We propose to isolate mutants of
these microorganisms that are missing caprolactone hydrolase enzyme activity.
Thus, the caprolactone that is formed from cyclohexane conversion by the
mutant microbes will accumulate and be excreted as a final end product. The
additional reducing power to regenerate the second NADPH cofactor will be
supplied by co-metabolism of acetate to CO2 and H20.
We propose that the microbial cells will be immobilized in a fixed bed
bioreactor and used as a biological catalyst for cyclohexane conversion. The
caprolactone that is produced in the bioreactor will be isolated and converted
directly into caprolactam, the nylon-6 precursor, by reaction with ammonia at
300*C and 300 atm. The direct conversion reaction is an established
AlliedSignal technology. In addition, the caprolactone itself can serve as a
monomer for polymerization. The polyester that is produced is a biodegradable
plastic.AlliedSignal
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St.Martin, E.J. A biological/chemical process for reduced waste and energy consumption, Caprolactam production: Phase 1, Select microorganisms and demonstrate feasibility. Final report, report, August 1, 1995; United States. (https://digital.library.unt.edu/ark:/67531/metadc791629/m1/14/: accessed April 24, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.