Crystallization of a member of the recFOR DNA repair pathway, RecO, with and without bound oligonucleotide

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RecFOR proteins are important for DNA repair by homologous recombination in bacteria. The RecO protein from Thermus thermophilus was cloned, purified and characterized for its binding to oligonucleotides. The protein was crystallized alone and in complex with a 14-mer oligonucleotide. Both crystal forms grow under different crystallization conditions in the same space group, P3121 or P3221, with almost identical unit cell parameters. Complete data sets were collected to 2.8 Angstrom and 2.5 Angstrom for RecO alone and the RecO-oligonucleotide complex, respectively. Visual comparison of the diffraction patterns between the two crystal forms and calculation of an Rmerge of 33.9 percent ... continued below

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Aono, Shelly; Hartsch, Thomas & Schulze-Gahmen, Ursula January 22, 2003.

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RecFOR proteins are important for DNA repair by homologous recombination in bacteria. The RecO protein from Thermus thermophilus was cloned, purified and characterized for its binding to oligonucleotides. The protein was crystallized alone and in complex with a 14-mer oligonucleotide. Both crystal forms grow under different crystallization conditions in the same space group, P3121 or P3221, with almost identical unit cell parameters. Complete data sets were collected to 2.8 Angstrom and 2.5 Angstrom for RecO alone and the RecO-oligonucleotide complex, respectively. Visual comparison of the diffraction patterns between the two crystal forms and calculation of an Rmerge of 33.9 percent on F indicate that one of the crystal forms is indeed a complex of RecO with bound oligonucleotide.

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  • Journal Name: Acta Crystallographica D; Journal Volume: 59; Journal Issue: pt 3; Other Information: Journal Publication Date: 03/2003

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  • Report No.: LBNL--52057
  • Grant Number: AC03-76SF00098
  • Office of Scientific & Technical Information Report Number: 823753
  • Archival Resource Key: ark:/67531/metadc789095

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  • January 22, 2003

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  • Dec. 3, 2015, 9:30 a.m.

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  • April 4, 2016, 12:35 p.m.

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Aono, Shelly; Hartsch, Thomas & Schulze-Gahmen, Ursula. Crystallization of a member of the recFOR DNA repair pathway, RecO, with and without bound oligonucleotide, article, January 22, 2003; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc789095/: accessed September 26, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.