THE BIOCATALYTIC DESULFURIZATION PROJECT

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The analysis of Petro Star diesel sulfur species is complete and a report is attached. Further analytical efforts will concentrate on characterization of diesel fuel, hydrodesulfurized to varying degrees, in order to determine sulfur species that may be problematic to hydrogen treatment and represent potential target substrates for biodesulfurization in a combined HDS-BDS process. Quotes have been received and are being considered for the partial treatment of Petro Star Inc. marine diesel fuel. Direction of research is changing slightly; economic analysis of the hyphenated--BDSHDS, BDS-CED--has shown the highest probability of success to be with a BDS-HDS process where the biodesulfurization ... continued below

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34 pages

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Collins, Scott & Nunn, David October 1, 2003.

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Description

The analysis of Petro Star diesel sulfur species is complete and a report is attached. Further analytical efforts will concentrate on characterization of diesel fuel, hydrodesulfurized to varying degrees, in order to determine sulfur species that may be problematic to hydrogen treatment and represent potential target substrates for biodesulfurization in a combined HDS-BDS process. Quotes have been received and are being considered for the partial treatment of Petro Star Inc. marine diesel fuel. Direction of research is changing slightly; economic analysis of the hyphenated--BDSHDS, BDS-CED--has shown the highest probability of success to be with a BDS-HDS process where the biodesulfurization precedes hydrodesulfurization. Thus, the microorganisms will be tailored to focus on those compounds that tend to be recalcitrant to hydrodesulfurization and decrease the severity of the hydrodesulfurization step. A separate, detailed justification for this change is being prepared. Research activities have continued in the characterization of the desulfurization enzymes from multiple sources. Genes for all DszA, -B, -C and -D enzymes (and homologs) have been cloned and expressed. Activity determinations, on a variety of substituted benzothiophene and dibenzothiophene substrates, have been carried out and continue. In addition, chemical synthesis efforts have been carried out to generate additional substrates for analytical standards and activity determinations. The generation of a GSSM mutant library of the ''Rhodococcus IGTS8 dszA'' gene has been completed and development of protocols for a high throughput screen to expand substrate specificity are nearing completion. In an effort to obtain improved hosts as biocatalyst, one hundred-thirty ''Rhodococcus'' and related strains are being evaluated for growth characteristics and other criteria deemed important for an optimal biocatalyst strain. We have also begun an effort to generate derivatives of the entire IGTS8 BDS plasmid that will allow for its easy transfer and manipulation into a variety of hosts. To support this activity and to gain an understanding of additional genes that may potentially affect BDS activity, the nucleotide sequence of the entire complement of plasmids in IGTS8 is being determined. Lastly, we continue to develop genetic screens and selections for the discovery and improvement of the biodesulfurization genes and strains.

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34 pages

Notes

OSTI as DE00822310

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  • Other Information: PBD: 1 Oct 2003

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  • Report No.: NONE
  • Grant Number: FC26-02NT15340
  • DOI: 10.2172/822310 | External Link
  • Office of Scientific & Technical Information Report Number: 822310
  • Archival Resource Key: ark:/67531/metadc788881

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • October 1, 2003

Added to The UNT Digital Library

  • Dec. 3, 2015, 9:30 a.m.

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  • Dec. 16, 2016, 4:45 p.m.

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Collins, Scott & Nunn, David. THE BIOCATALYTIC DESULFURIZATION PROJECT, report, October 1, 2003; United States. (digital.library.unt.edu/ark:/67531/metadc788881/: accessed May 25, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.