A saturation screen for cis-acting regulatory DNA in the Hox genes of Ciona intestinalis

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A screen for the systematic identification of cis-regulatory elements within large (>100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis. Randomly generated DNA fragments from bacterial artificial chromosomes containing two clusters of Hox genes were inserted into a vector upstream of a minimal promoter and lacZ reporter gene. A total of 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulatory activities were monitored in larvae. In sum, 21 separable cis-regulatory elements were found. These include eight Hox linked domains that drive expression in nested anterior-posterior domains of ectodermally derived ... continued below

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Keys, David N.; Lee, Byung-in; Di Gregorio, Anna; Harafuji, Naoe; Detter, Chris; Wang, Mei et al. January 5, 2005.

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A screen for the systematic identification of cis-regulatory elements within large (>100 kb) genomic domains containing Hox genes was performed by using the basal chordate Ciona intestinalis. Randomly generated DNA fragments from bacterial artificial chromosomes containing two clusters of Hox genes were inserted into a vector upstream of a minimal promoter and lacZ reporter gene. A total of 222 resultant fusion genes were separately electroporated into fertilized eggs, and their regulatory activities were monitored in larvae. In sum, 21 separable cis-regulatory elements were found. These include eight Hox linked domains that drive expression in nested anterior-posterior domains of ectodermally derived tissues. In addition to vertebrate-like CNS regulation, the discovery of cis-regulatory domains that drive epidermal transcription suggests that C. intestinalis has arthropod-like Hox patterning in the epidermis.

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OSTI as DE00840869

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  • Journal Name: Proceedings of the National Academy of Sciences of the United States of America; Journal Volume: 102; Journal Issue: 3; Other Information: Submitted to the Proceedings of the National Academy of Sciences of the United States of America: Volume 102, No.3; Journal Publication Date: 01/18/2005

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  • Report No.: LBNL--49910
  • Grant Number: AC03-76SF00098
  • Office of Scientific & Technical Information Report Number: 840869
  • Archival Resource Key: ark:/67531/metadc788784

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  • January 5, 2005

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  • Dec. 3, 2015, 9:30 a.m.

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  • April 4, 2016, 3:38 p.m.

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Keys, David N.; Lee, Byung-in; Di Gregorio, Anna; Harafuji, Naoe; Detter, Chris; Wang, Mei et al. A saturation screen for cis-acting regulatory DNA in the Hox genes of Ciona intestinalis, article, January 5, 2005; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc788784/: accessed October 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.