A system for combined three-dimensional morphological and molecular analysis of thick tissue specimens

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We present a new system for simultaneous morphological and molecular analysis of thick tissue samples. The system is composed of a computer assisted microscope and a JAVA-based image display, analysis and visualization program that allows acquisition, annotation, meaningful storage, three-dimensional reconstruction and analysis of structures of interest in thick sectioned tissue specimens. We describe the system in detail and illustrate its use by imaging, reconstructing and analyzing two complete tissue blocks which were differently processed and stained. One block was obtained from a ductal carcinoma in situ (DCIS) lumpectomy specimen and stained alternatively with Hematoxilyn and Eosin (H&E), and with ... continued below

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Fernandez-Gonzalez, Rodrigo; Jones, Arthur; Garcia-Rodriguez, Enrique; Yuan Chen, Ping; Idica, Adam; Lockett, Stephen J. et al. April 25, 2002.

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We present a new system for simultaneous morphological and molecular analysis of thick tissue samples. The system is composed of a computer assisted microscope and a JAVA-based image display, analysis and visualization program that allows acquisition, annotation, meaningful storage, three-dimensional reconstruction and analysis of structures of interest in thick sectioned tissue specimens. We describe the system in detail and illustrate its use by imaging, reconstructing and analyzing two complete tissue blocks which were differently processed and stained. One block was obtained from a ductal carcinoma in situ (DCIS) lumpectomy specimen and stained alternatively with Hematoxilyn and Eosin (H&E), and with a counterstain and fluorescence in situ hybridization (FISH) to the ERB-B2 gene. The second block contained a fully sectioned mammary gland of a mouse, stained for Histology with H&E. We show how the system greatly reduces the amount of interaction required for the acquisition and analysis and is therefore suitable for studies that require morphologically driven, wide scale (e.g., whole gland) analysis of complex tissue samples or cultures.

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  • Journal Name: Microscopy Research and Technique; Journal Volume: 59; Journal Issue: 6; Other Information: Journal Publication Date: 12/15/2002

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  • Report No.: LBNL--50261
  • Grant Number: AC03-76SF00098
  • DOI: 10.1002/jemt.10233 | External Link
  • Office of Scientific & Technical Information Report Number: 823277
  • Grant Number: DAMD17-00-1-0306
  • Grant Number: DAMD17-00-1-0227
  • Archival Resource Key: ark:/67531/metadc787318

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  • April 25, 2002

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  • Dec. 3, 2015, 9:30 a.m.

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  • Nov. 30, 2017, 7:09 p.m.

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Fernandez-Gonzalez, Rodrigo; Jones, Arthur; Garcia-Rodriguez, Enrique; Yuan Chen, Ping; Idica, Adam; Lockett, Stephen J. et al. A system for combined three-dimensional morphological and molecular analysis of thick tissue specimens, article, April 25, 2002; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc787318/: accessed September 25, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.