Reduction and Methyl Transfer Kinetics of the Alpha Subunit from Acetyl-Coenzyme A Synthase

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OAK-B135 Stopped-flow was used to evaluate the methylation and reduction kinetics of the isolated alpha subunit of acetyl-Coenzyme A synthase from Moorella thermoacetica. This catalytically active subunit contains a novel Ni-X-Fe4S4 cluster and a putative unidentified n =2 redox site called D. The D-site must be reduced for a methyl group to transfer from a corrinoid-iron-sulfur protein, a key step in the catalytic synthesis of acetyl-CoA. The Fe4S4 component of this cluster is also redox active, raising the possibility that it is the D-site or a portion thereof. Results presented demonstrate that the D-site reduces far faster than the Fe4S4 ... continued below

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Tan, Xiangshi; Sewell, Christopher; Yang, Qingwu & Lindahl, Paul A. January 15, 2003.

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OAK-B135 Stopped-flow was used to evaluate the methylation and reduction kinetics of the isolated alpha subunit of acetyl-Coenzyme A synthase from Moorella thermoacetica. This catalytically active subunit contains a novel Ni-X-Fe4S4 cluster and a putative unidentified n =2 redox site called D. The D-site must be reduced for a methyl group to transfer from a corrinoid-iron-sulfur protein, a key step in the catalytic synthesis of acetyl-CoA. The Fe4S4 component of this cluster is also redox active, raising the possibility that it is the D-site or a portion thereof. Results presented demonstrate that the D-site reduces far faster than the Fe4S4 component, effectively eliminating this possibility. Rather, this component may alter catalytically important properties of the Ni center. The D-site is reduced through a pathway that probably does not involve the Fe4S4 component of this active-site cluster.

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OSTI as DE00826207

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  • Journal Name: Journal of the American Chemical Society; Journal Volume: 125; Journal Issue: 2; Other Information: Printed in the Journal of the American Chemical Society, Volume 125, No.2; DOI 10.1021/ja028442t

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  • Report No.: DOE/ER/15177-1
  • Grant Number: FG03-01ER15177
  • DOI: 10.1021/ja028442t | External Link
  • Office of Scientific & Technical Information Report Number: 826207
  • Archival Resource Key: ark:/67531/metadc787118

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  • January 15, 2003

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  • Dec. 3, 2015, 9:30 a.m.

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  • Jan. 4, 2017, 2:01 p.m.

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Tan, Xiangshi; Sewell, Christopher; Yang, Qingwu & Lindahl, Paul A. Reduction and Methyl Transfer Kinetics of the Alpha Subunit from Acetyl-Coenzyme A Synthase, article, January 15, 2003; United States. (digital.library.unt.edu/ark:/67531/metadc787118/: accessed December 11, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.