Magnetic bead purification of labeled DNA fragments forhigh-throughput capillary electrophoresis sequencing

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We have developed an automated purification method for terminator sequencing products based on a magnetic bead technology. This 384-well protocol generates labeled DNA fragments that are essentially free of contaminates for less than $0.005 per reaction. In comparison to laborious ethanol precipitation protocols, this method increases the phred20 read length by forty bases with various DNA templates such as PCR fragments, Plasmids, Cosmids and RCA products. Our method eliminates centrifugation and is compatible with both the MegaBACE 1000 and ABIPrism 3700 capillary instruments. As of September 2001, this method has produced over 1.6 million samples with 93 percent averaging 620 ... continued below

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Elkin, Christopher; Kapur, Hitesh; Smith, Troy; Humphries, David; Pollard, Martin; Hammon, Nancy et al. September 15, 2001.

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We have developed an automated purification method for terminator sequencing products based on a magnetic bead technology. This 384-well protocol generates labeled DNA fragments that are essentially free of contaminates for less than $0.005 per reaction. In comparison to laborious ethanol precipitation protocols, this method increases the phred20 read length by forty bases with various DNA templates such as PCR fragments, Plasmids, Cosmids and RCA products. Our method eliminates centrifugation and is compatible with both the MegaBACE 1000 and ABIPrism 3700 capillary instruments. As of September 2001, this method has produced over 1.6 million samples with 93 percent averaging 620 phred20 bases as part of Joint Genome Institutes Production Process.

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  • Journal Name: Biotechniques; Journal Volume: 32; Journal Issue: 6; Related Information: Journal Publication Date: June 2002

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  • Report No.: LBNL--48920
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 860368
  • Archival Resource Key: ark:/67531/metadc785896

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • September 15, 2001

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  • Dec. 3, 2015, 9:30 a.m.

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  • April 1, 2016, 8:18 p.m.

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Elkin, Christopher; Kapur, Hitesh; Smith, Troy; Humphries, David; Pollard, Martin; Hammon, Nancy et al. Magnetic bead purification of labeled DNA fragments forhigh-throughput capillary electrophoresis sequencing, article, September 15, 2001; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc785896/: accessed October 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.