Single-Cell Nonphotochemical Hole Burning of Ovarian Surface Epithelial Carcinoma and Normal Cells

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Persistent spectral nonphotochemical hole-burning (NPHB) spectroscopy has recently been applied to dye molecules in cells. The sensitivity of NPHB to the nanoenvironment of the probe is well established. It has been shown that NPHB applied to bulk suspensions of cultured human cells can distinguish between normal and cancer cells. Thus, NPHB has potential as a diagnostic cancer tool. For this reason, the methodology is referred to as hole-burning imaging, by analogy with MRI. The optical dephasing time (T{sub 2}) of the dye in hole-burning image replaces the proton T{sub 1} relaxation time in MRI. In addition to the T{sub 2} ... continued below

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Walsh, R.J.; Matsuzaki, S.; Reinot, T.; Hayes, J.M.; Kalli, K.R.; Hartmann, L.C. et al. September 30, 2004.

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Persistent spectral nonphotochemical hole-burning (NPHB) spectroscopy has recently been applied to dye molecules in cells. The sensitivity of NPHB to the nanoenvironment of the probe is well established. It has been shown that NPHB applied to bulk suspensions of cultured human cells can distinguish between normal and cancer cells. Thus, NPHB has potential as a diagnostic cancer tool. For this reason, the methodology is referred to as hole-burning imaging, by analogy with MRI. The optical dephasing time (T{sub 2}) of the dye in hole-burning image replaces the proton T{sub 1} relaxation time in MRI. In addition to the T{sub 2} mode of operation, there are four other modes including measurement of the spectral hole growth kinetics (HGK). Reported here is that the selectivity and sensitivity of NPHB operating in the HGK mode allow for distinction between normal and carcinoma cells at the single-cell level. The ovarian cell lines are ovarian surface epithelial cells with temperature-sensitivity large T antigens (analogously normal) and ovarian surface epithelial carcinoma (OV167) cells. The mitochondrial specific dye used was rhodamine 800 (Molecular Probes). This carbocationic dye is highly specific for the outer and inner membranes of mitochondria. In line with the results for bulk suspensions of the two cell lines, the hole-burning efficiency for OV167 cells was found to be significantly higher than that for normal cells. Theoretical analysis of the HGK data leads to the conclusion that the degree of structural heterogeneity for the probe-host configurations in OV167 cells is lower than in the normal cells. Possible reasons for this are given.

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INIS; OSTI as DE00832899

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  • Report No.: IS-M 925
  • Grant Number: W-7405-Eng-82
  • Office of Scientific & Technical Information Report Number: 832899
  • Archival Resource Key: ark:/67531/metadc784828

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  • September 30, 2004

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  • Dec. 3, 2015, 9:30 a.m.

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  • Jan. 2, 2018, 3:26 p.m.

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Walsh, R.J.; Matsuzaki, S.; Reinot, T.; Hayes, J.M.; Kalli, K.R.; Hartmann, L.C. et al. Single-Cell Nonphotochemical Hole Burning of Ovarian Surface Epithelial Carcinoma and Normal Cells, article, September 30, 2004; Ames, Iowa. (digital.library.unt.edu/ark:/67531/metadc784828/: accessed October 17, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.