Natural and Passive Remediation of Chlorinated Solvents: Critical Evaluation of Science and Technology Targets Page: 97 of 150

                                
WSRC-TR-2003-00328
February 19, 2004

Table 2. High Priority Technical and Implementation Targets Recommended for US DOE
MNA/EPR Alternative Project

Direct measures of Attenuation Mechanisms
a) Develop advanced bioassessment tools for determining ongoing and
potential microbial processes at different sites.
- includes reductive processes (Dehalococcoides etc.)
- new methods to quantify growth.
- gene sequences used to develop molecular probes for micro arrays and
quantitative PCR.
- additional key functional genes for use in Microarrays
- additional key functional genes for use in Microarrays
- genes whose expression correlates with specific environmental conditions
- DNA microarrays used to simultaneously monitor the expression of many
genes

b) Develop field scale correlations between species, functional genes, and  $$
degradation rate and potential
- Laboratory and field tests to develop and correlate parameters
- Assess factors that would bias the application of these molecular tools
c) Conduct further research on oxidative and reductive processes (several  $$
specific sub-needs listed)
d) Develop direct measures and sensors for abiotic attenuation        $
mechanisms. e.g. resistive fraction; develop method to measure; results could
be used to establish capacity as well as provide concentration at which to
transition from active treatments
Strategies to Support Characterization and Monitoring
e) Develop a scenarios based framework to support characterization    $
monitoring and modeling decisionmaking
- Taxonomic key to help define multiple lines of evidence.
f) Develop specific alternative monitoring configurations to collect data at $
substantial cost savings during system performance monitoring stage
- Examples include horizontal wells to replace several vertical wells.
g) Improve integration of modeling into MNA evaluation and            $
implementation process
h) Improve reaction forms and include variable linear equilibrium     $
partitioning coefficients within analytical models
Tools to Support Characterization and Monitoring
i) Develop threshold and binary monitoring devices and strategies for  $
long-term monitoring

84

Cost

$$