Effect of secondary structure on the potential of mean force for poly-L-lysine in the alpha-Helix and beta-sheet conformations Metadata

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Title

  • Main Title Effect of secondary structure on the potential of mean force for poly-L-lysine in the alpha-Helix and beta-sheet conformations

Creator

  • Author: Grigsby, J.J.
    Creator Type: Personal
  • Author: Blanch, H.W.
    Creator Type: Personal
  • Author: Prausnitz, J.M.
    Creator Type: Personal

Contributor

  • Sponsor: USDOE Director, Office of Science. Office of Basic Energy Sciences. Division of Chemical Sciences
    Contributor Type: Organization
  • Sponsor: National Science Foundation (United States)
    Contributor Type: Organization

Publisher

  • Name: Lawrence Berkeley National Laboratory
    Place of Publication: Berkeley, California
    Additional Info: Ernest Orlando Lawrence Berkeley National Laboratory, Berkeley, CA (United States)

Date

  • Creation: 2001-10-30

Language

  • English

Description

  • Content Description: Because poly-L-lysine (PLL) can exist in the {alpha}-helix or {beta}-sheet conformation depending on solution preparation and solution conditions, PLL is a suitable candidate to probe the dependence of protein interactions on secondary structure. The osmotic second virial coefficient and weight-average molecular weight are reported from low-angle laser-light scattering measurements for PLL as a function of NaCl concentration, pH, and {alpha}-helix or {beta}-sheet content. Interactions between PLL molecules become more attractive as salt concentration increases due to screening of PLL charge by salt ions and at low salt concentration become more attractive as pH increases due to decreased net charge on PLL. The experimental results show that interactions are stronger for the {beta}-sheet conformation than for the {alpha}-helix conformation. A spherically-symmetric model for the potential of mean force is used to account for specific interactions not described by DLVO theory and to show how differences in secondary structure affect PLL interactions.
  • Physical Description: 24 pages

Subject

  • STI Subject Categories: 37 Inorganic, Organic, Physical And Analytical Chemistry
  • Keyword: Proteins
  • STI Subject Categories: 59 Basic Biological Sciences
  • Keyword: Scattering
  • Keyword: Probes
  • Keyword: Molecular Weight

Source

  • Journal Name: Biophysical Chemistry; Journal Volume: 99; Journal Issue: 2; Other Information: Submitted to Biophysical Chemistry: Volume 99, No.2; Journal Publication Date: 10/16/2002

Collection

  • Name: Office of Scientific & Technical Information Technical Reports
    Code: OSTI

Institution

  • Name: UNT Libraries Government Documents Department
    Code: UNTGD

Resource Type

  • Article

Format

  • Text

Identifier

  • Report No.: LBNL--49230
  • Grant Number: AC03-76SF00098
  • Office of Scientific & Technical Information Report Number: 837911
  • Archival Resource Key: ark:/67531/metadc783496

Note

  • Display Note: OSTI as DE00837911
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