Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport

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This article discusses functional characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter.

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12 p.

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Srivastava, Avinash C.; Ganesan, Savita; Ismail, Ihab O. & Ayre, Brian G. September 2008.

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This article discusses functional characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter.

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12 p.

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Plant Physiology, September 2008, Vol. 148, pp. 200-211, www.plantphysiol.org/cgi/doi/10.1104/pp.108.124776; © American Society of Plant Biologists

Abstract: AtSUC2 (At1g22710) encodes a phloem-localized sucrose (Suc)/H+ symporter necessary for efficient Suc transport from source tissues to sink tissues in Arabidopsis (Arabidopsis thaliana). AtSUC2, however, is also expressed strongly in the transport phloem, where its role is more ambiguous, and it has been implicated in mediating both efflux and retrieval to and from flanking tissues via the apoplast. To characterize the role of AtSUC2 in controlling carbon partitioning along the phloem path, AtSUC2 cDNA was expressed from tissue-specific promoters in an Atsuc2 mutant background. Suc transport in this mutant is highly compromised, as indicated by stunted growth and the accumulation of large quantities of sugar and starch in vegetative tissues. Expression of AtSUC2 cDNA from the 2-kb AtSUC2 promoter was sufficient to restore growth and carbon partitioning to nearly wild-type levels. The GALACTINOL SYNTHASE promoter of Cucumis melo (CmGAS1p) confers expression only in the minor veins of mature leaves, not in the transport phloem of larger leaf veins and stems. Mutant plants expressing AtSUC2 cDNA from CmGAS1p had intermediate growth and accumulated sugar and starch, but otherwise they had normal morphology. These characteristics support a role for AtSUC2 in retrieval but not efflux along the transport phloem and show that the only vital function of AtSUC2 in photoassimilate distribution is phloem loading. In addition, AtSUC2 mutant plants, although debilitated, do grow, and AtSUC2-independent modes of phloem transport are discussed, including an entirely symplastic pathway from mesophyll cells to sink tissues.

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  • Plant Physiology, 2008, Rockville: American Society of Plant Biologists, pp. 200-211

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  • Publication Title: Plant Physiology
  • Volume: 148
  • Page Start: 200
  • Page End: 211
  • Peer Reviewed: Yes

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UNT Scholarly Works

The Scholarly Works Collection is home to materials from the University of North Texas community's research, creative, and scholarly activities and serves as UNT's Open Access Repository. It brings together articles, papers, artwork, music, research data, reports, presentations, and other scholarly and creative products representing the expertise in our university community. Access to some items in this collection may be restricted.

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  • September 2008

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  • April 2, 2012, 4:46 p.m.

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  • July 22, 2013, 11:51 a.m.

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Srivastava, Avinash C.; Ganesan, Savita; Ismail, Ihab O. & Ayre, Brian G. Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport, article, September 2008; [Rockville, Maryland]. (digital.library.unt.edu/ark:/67531/metadc78290/: accessed April 27, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.