Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene

Srivastava, Avinash C.; Ganesan, Savita; Ismail, Ihab O.; Ayre, Brian G.

doi:10.1186/1471-2229-9-7

Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene Metadata

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Title

Main Title Effective carbon partitioning driven by exotic phloem-specific regulatory elements fused to the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene

Creator

Author: Srivastava, Avinash C.

Creator Type: Personal

Creator Info: University of North Texas; Samuel Roberts Noble Foundation
Author: Ganesan, Savita

Creator Type: Personal

Creator Info: University of North Texas; Amyris Biotechnologies, Inc.
Author: Ismail, Ihab O.

Creator Type: Personal

Creator Info: University of North Texas
Author: Ayre, Brian G.

Creator Type: Personal

Creator Info: University of North Texas

Publisher

Name: BioMed Central Ltd.

Place of Publication: [London, United Kingdom]

Date

Creation: 2009-01-20

Language

English

Description

Content Description: This article discusses the effect and overall importance of the Arabidopsis thaliana AtSUC2 sucrose-proton symporter gene on carbon partitioning and plant growth.
Physical Description: 13 p.

Subject

Keyword: carbon partitioning
Keyword: sucrose-proton symporter
Keyword: genes

Source

Journal: BMC Plant Biology, 9(7), BioMed Central, January 20, 2009, pp. 1-13

Citation

Publication Title: BMC Plant Biology
Volume: 9
Issue: 7
Peer Reviewed: True

Collection

Name: UNT Scholarly Works

Code: UNTSW

Institution

Name: UNT College of Arts and Sciences

Code: UNTCAS

Rights

Rights Access: public
Rights License: by

Resource Type

Article

Format

Text

Identifier

DOI: 10.1186/1471-2229-9-7
Archival Resource Key: ark:/67531/metadc78288

Degree

Academic Department: Biological Sciences

Note

Display Note: Abstract: Background: AtSUC2 (At1g22710) from Arabidopsis thaliana encodes a phloem-localized sucrose/proton symporter required for efficient photoassimilate transport from source tissues to sink tissues. AtSUC2 plays a key role in coordinating the demands of sink tissues with the output capacity of source leaves, and in maintaining phloem hydrostatic pressure during changes in plant-water balance. Expression and activity are regulated, both positively and negatively, by developmental (sink or source transition) and environmental cues, including light, diurnal changes, photoassimilate levels, turgor pressure, drought and osmotic stress, and hormones. Results: To assess the importance of this regulation to whole-plant growth and carbon partitioning, AtSUC2 cDNA was expressed from two exotic, phloem-specific promoters in a mutant background debilitated for AtSUC2 function. The first was a promoter element from Commelina Yellow Mottle Virus (CoYMV), and the second was the rolc promoter from Agrobacterium rhizogenes. CoYMVp::AtSUC2 cDNA restored growth and carbon partitioning to near wild-type levels, whereas plants harboring rolCp::AtSUC2 cDNA showed only partial complementation. Conclusion: Expressing AtSUC2 cDNA from exotic, phloem-specific promoters argues that strong, phloem-localized expression is sufficient for efficient transport. Expressing AtSUC2 from promoters that foster efficient phloem transport but are subject to regulatory cascades different from the endogenous sucrose/proton symporter genes has implications for biotechnology.