Gene expression profiles of Nitrosomonas europaea, an obligate chemolitotroph Page: 3 of 5
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number of TonB-dependent Fe-siderophore outer membrane receptors were the proteins in the
predominant bands (Table 2). These proteins include at least six TonB-dependent outer membrane
Fe/siderophore receptors that can be potentially involved in Fe uptake. All these six Fe/siderophore
receptor genes are those that are not associated with ECF o-factor and sensor genes. As shown in Table
2, some of them are similar to ferrichrome receptor (NE 1089), or catechol/catecholate (enterobactin,
NE1540) receptor, but four out of six are unidentified siderophore receptors. This result provides direct
evidence for the essential role of the genes and their protein products in Fe acquisition and hence the
growth and survival of N. europaea under Fe-limiting conditions. Interestingly, an outer membrane
protein OmpC (a general diffusion Gram-negative porin) and a type II secretion pathway protein were
also identified in the protein bands. These proteins have molecular weights around 78 and 82 kDa.
N. europaea can adapt to low Fe growth environment. Higher expression of these Fe acquisition related
proteins in cells grown in low Fe medium provides further biochemical evidence for the adaptation of
N. europaea to Fe limiting conditions.
Genes encoding the putative outer membrane desferal receptor (NE1097 and NE1088,foxA
homologues) have been cloned, insertional mutant constructs made, and mutant strains obtained through
homologous recombination. We have already confirmed that desferal receptor gene is essential for the
bacterium to utilize Fe-loaded desferal. Further physiological and genetic characterization of these
mutants is in progress (unpublished and reported at DOE Genomics: GTL. Contractor-Grantee Workshop
III. Washington, D.C. February 6-9, 2005).
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Arp, Daniel J. Gene expression profiles of Nitrosomonas europaea, an obligate chemolitotroph, report, May 25, 2005; Corvallis, Oregon. (https://digital.library.unt.edu/ark:/67531/metadc782050/m1/3/: accessed April 19, 2024), University of North Texas Libraries, UNT Digital Library, https://digital.library.unt.edu; crediting UNT Libraries Government Documents Department.