Microbial Transformations of TRU and Mixed Wastes: Actinide Speciation and Waste Volume Reduction

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Work has been performed in two areas; the reduction of uranium by bacteria and the characterization of cellulose degraded by bacteria. The uranium reduction study utilized Shewanella oneidensis, a widely distributed species of bacteria known to utilize several elements such as iron, manganese and sulfur as electron acceptors. Shewanella oneidensis was grown aerobically approximately 24 hours at room temperature in Tryptic Soy Broth (TSB). Cells are then concentrated by centrifugation and washed thoroughly with NaHCO3 (2.5g/L) buffer. A small sub-sample of cells are stained with DAPI and counted under a microscope for accurate determination of cell number. The cells are ... continued below

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Francis, Arokiasamy J.; Halada, Gary P. & Czerwinski, K. June 1, 2003.

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Description

Work has been performed in two areas; the reduction of uranium by bacteria and the characterization of cellulose degraded by bacteria. The uranium reduction study utilized Shewanella oneidensis, a widely distributed species of bacteria known to utilize several elements such as iron, manganese and sulfur as electron acceptors. Shewanella oneidensis was grown aerobically approximately 24 hours at room temperature in Tryptic Soy Broth (TSB). Cells are then concentrated by centrifugation and washed thoroughly with NaHCO3 (2.5g/L) buffer. A small sub-sample of cells are stained with DAPI and counted under a microscope for accurate determination of cell number. The cells are then diluted in NaHCO3 to the appropriate concentration of 2*108 cells/mL. No cells were added as a control. A 5mL solution of cells is then transferred into 100mL of sterile anaerobic bicarbonate buffered freshwater medium with the phosphate removed and 5mM lactate as the carbon source. At several time points three 1mL samples are removed from each of the batch experiments and the control into 1.5mL eppendorf tubes containing 0.1mL formaldehyde. The samples are then removed from the anaerobic environment and frozen until analysis. Individual samples are filtered (0.2 micron pore size) to remove any uraninite and cellular material and diluted with 4mL 0.1M HNO3. The concentration of uranyl present in each sample was measured using UV-Visible spectroscopy. Data are fit to a first order model as follows: [U]t=[U]oe{sup -}kt + [U]eq where [U]o is the initial uranyl solution concentration, [U]t is the uranyl solution concentration at time t, and [U]eq is the uranyl concentration remaining in solution at equilibrium. The use of the first order fit was based on previous studies and publications.

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  • Other Information: PBD: 1 Jun 2003

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  • Report No.: EMSP-90258--2003
  • DOI: 10.2172/839349 | External Link
  • Office of Scientific & Technical Information Report Number: 839349
  • Archival Resource Key: ark:/67531/metadc781256

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  • June 1, 2003

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  • Dec. 3, 2015, 9:30 a.m.

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  • Oct. 4, 2016, 11:24 a.m.

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Francis, Arokiasamy J.; Halada, Gary P. & Czerwinski, K. Microbial Transformations of TRU and Mixed Wastes: Actinide Speciation and Waste Volume Reduction, report, June 1, 2003; Upton, New York. (digital.library.unt.edu/ark:/67531/metadc781256/: accessed September 24, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.