Tracking chemical changes in a live cell: Biomedical applications of SR-FTIR spectromicroscopy

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Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05 - 0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and ... continued below

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Holman, Hoi-Ying N.; Martin, Michael C. & McKinney, Wayne R. July 25, 2002.

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Synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectromicroscopy is a newly emerging bioanalytical and imaging tool. This unique technique provides mid-infrared (IR) spectra, hence chemical information, with high signal-to-noise at spatial resolutions as fine as 3 to 10 microns. Thus it enables researchers to locate, identify, and track specific chemical events within an individual living mammalian cell. Mid-IR photons are too low in energy (0.05 - 0.5 eV) to either break bonds or to cause ionization. In this review, we show that the synchrotron IR beam has no detectable effects on the short- and long-term viability, reproductive integrity, cell-cycle progression, and mitochondrial metabolism in living human cells, and produces only minimal sample heating (< 0.5 degrees C). We will then present several examples demonstrating the application potentials of SR-FTIR spectromicroscopy in biomedical research. These will include monitoring living cells progressing through the cell cycle, including death, and cells reacting to dilute concentrations of toxins.

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INIS; OSTI as DE00834624

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  • First International Conference on Biomedical Spectroscopy. From Molecules to Men, Cardiff, Wales (GB), 07/07/2002--07/10/2002

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  • Report No.: LBNL--51337
  • Grant Number: AC03-76SF00098
  • Office of Scientific & Technical Information Report Number: 834624
  • Archival Resource Key: ark:/67531/metadc780692

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  • July 25, 2002

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  • Dec. 3, 2015, 9:30 a.m.

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  • April 4, 2016, 5:44 p.m.

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Holman, Hoi-Ying N.; Martin, Michael C. & McKinney, Wayne R. Tracking chemical changes in a live cell: Biomedical applications of SR-FTIR spectromicroscopy, article, July 25, 2002; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc780692/: accessed October 19, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.