Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences

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Description

The major objective of the research was to provide appropriate sequences and to assemble a DNA arrays of oligonucleotides to be used for rapid profiling of microbial populations, from polluted areas and from areas of other interest. The sequences to be assigned to the DNA array are chosen from cloned genomic DNA from groundwater at DOE sites containing organic solvents. The sites, Hanford Nuclear Plant and Lawrence Livermore Site 300 (LLNL), have well characterized pollutant histories, which have been provided by our collaborators.

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Robb, Frank T. June 1, 2000.

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Description

The major objective of the research was to provide appropriate sequences and to assemble a DNA arrays of oligonucleotides to be used for rapid profiling of microbial populations, from polluted areas and from areas of other interest. The sequences to be assigned to the DNA array are chosen from cloned genomic DNA from groundwater at DOE sites containing organic solvents. The sites, Hanford Nuclear Plant and Lawrence Livermore Site 300 (LLNL), have well characterized pollutant histories, which have been provided by our collaborators.

Physical Description

vp.

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  • Other Information: PBD: 1 Jun 2000

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  • Report No.: EMSP-55152--2000
  • Grant Number: FG07-96ER62320
  • DOI: 10.2172/827425 | External Link
  • Office of Scientific & Technical Information Report Number: 827425
  • Archival Resource Key: ark:/67531/metadc780603

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • June 1, 2000

Added to The UNT Digital Library

  • Dec. 3, 2015, 9:30 a.m.

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  • April 21, 2016, 8:47 p.m.

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Robb, Frank T. Molecular Profiling of Microbial Communities from Contaminated Sources: Use of Subtractive Cloning Methods and rDNA Spacer Sequences, report, June 1, 2000; United States. (digital.library.unt.edu/ark:/67531/metadc780603/: accessed October 18, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.