Sequencing and comparing whole mitochondrial genomes ofanimals

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Comparing complete animal mitochondrial genome sequences is becoming increasingly common for phylogenetic reconstruction and as a model for genome evolution. Not only are they much more informative than shorter sequences of individual genes for inferring evolutionary relatedness, but these data also provide sets of genome-level characters, such as the relative arrangements of genes, that can be especially powerful. We describe here the protocols commonly used for physically isolating mtDNA, for amplifying these by PCR or RCA, for cloning,sequencing, assembly, validation, and gene annotation, and for comparing both sequences and gene arrangements. On several topics, we offer general observations based on ... continued below

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Boore, Jeffrey L.; Macey, J. Robert & Medina, Monica April 22, 2005.

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Comparing complete animal mitochondrial genome sequences is becoming increasingly common for phylogenetic reconstruction and as a model for genome evolution. Not only are they much more informative than shorter sequences of individual genes for inferring evolutionary relatedness, but these data also provide sets of genome-level characters, such as the relative arrangements of genes, that can be especially powerful. We describe here the protocols commonly used for physically isolating mtDNA, for amplifying these by PCR or RCA, for cloning,sequencing, assembly, validation, and gene annotation, and for comparing both sequences and gene arrangements. On several topics, we offer general observations based on our experiences to date with determining and comparing complete mtDNA sequences.

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  • Report No.: LBNL--55278
  • Grant Number: DE-AC02-05CH11231
  • Office of Scientific & Technical Information Report Number: 861135
  • Grant Number: DEB-0089624
  • Grant Number: EAR-0120646
  • Grant Number: DEB-9807100
  • Archival Resource Key: ark:/67531/metadc778743

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • April 22, 2005

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  • Dec. 3, 2015, 9:30 a.m.

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  • Nov. 30, 2017, 7:10 p.m.

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Boore, Jeffrey L.; Macey, J. Robert & Medina, Monica. Sequencing and comparing whole mitochondrial genomes ofanimals, book, April 22, 2005; (digital.library.unt.edu/ark:/67531/metadc778743/: accessed April 19, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.