High-throughput film-densitometry: An efficient approach to generate large data sets

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Description

A film-handling machine (robot) has been built which can, in conjunction with a commercially available film densitometer, exchange and digitize over 300 electron micrographs per day. Implementation of robotic film handling effectively eliminates the delay and tedium associated with digitizing images when data are initially recorded on photographic film. The modulation transfer function (MTF) of the commercially available densitometer is significantly worse than that of a high-end, scientific microdensitometer. Nevertheless, its signal-to-noise ratio (S/N) is quite excellent, allowing substantial restoration of the output to ''near-to-perfect'' performance. Due to the large area of the standard electron microscope film that can be ... continued below

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33 pages; OS: Windows 2000

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Typke, Dieter; Nordmeyer, Robert A.; Jones, Arthur; Lee, Juyoung; Avila-Sakar, Agustin; Downing, Kenneth H. et al. July 14, 2004.

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Description

A film-handling machine (robot) has been built which can, in conjunction with a commercially available film densitometer, exchange and digitize over 300 electron micrographs per day. Implementation of robotic film handling effectively eliminates the delay and tedium associated with digitizing images when data are initially recorded on photographic film. The modulation transfer function (MTF) of the commercially available densitometer is significantly worse than that of a high-end, scientific microdensitometer. Nevertheless, its signal-to-noise ratio (S/N) is quite excellent, allowing substantial restoration of the output to ''near-to-perfect'' performance. Due to the large area of the standard electron microscope film that can be digitized by the commercial densitometer (up to 10,000 x 13,680 pixels with an appropriately coded holder), automated film digitization offers a fast and inexpensive alternative to high-end CCD cameras as a means of acquiring large amounts of image data in electron microscopy.

Physical Description

33 pages; OS: Windows 2000

Notes

OSTI as DE00836982

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  • Journal Name: Journal of Structural Biology; Journal Volume: 149; Journal Issue: 1; Other Information: Submitted to Journal of Structural Biology: Volume 149, No.1; Journal Publication Date: 01/2005

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  • Report No.: LBNL-56128
  • Grant Number: AC03-76SF00098
  • Office of Scientific & Technical Information Report Number: 836982
  • Grant Number: GM 62989
  • Archival Resource Key: ark:/67531/metadc777029

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • July 14, 2004

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  • Dec. 3, 2015, 9:30 a.m.

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  • July 26, 2016, 5:51 p.m.

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Typke, Dieter; Nordmeyer, Robert A.; Jones, Arthur; Lee, Juyoung; Avila-Sakar, Agustin; Downing, Kenneth H. et al. High-throughput film-densitometry: An efficient approach to generate large data sets, article, July 14, 2004; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc777029/: accessed December 17, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.