Genome-wide mutagenesis of Zea mays L. using RescueMu transposons

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This article discusses genome-wide mutagenesis of Zea mays L. using RescueMu transposons.

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20 p.

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Fernandes, John; Dong, Qunfeng; Schneider, Bret; Morrow, Darren J.; Nan, Guo-Ling; Brendel, Volker et al. 2004.

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This article is part of the collection entitled: UNT Scholarly Works and was provided by UNT College of Arts and Sciences to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 413 times . More information about this article can be viewed below.

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This article discusses genome-wide mutagenesis of Zea mays L. using RescueMu transposons.

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20 p.

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Abstract: Derived from the maize Mu I transposon, RescueMu provides strategies for maize gene discovery and mutant phenotypic analysis. 9.92 Mb of gene-enriched sequences next to RescueMu insertion sites were co-assembled with expressed sequence tags and analyzed. Multiple plasmid recoveries identified probable germinal insertions and screening of RescueMu plasmid libraries identified plants containing probable germinal insertions. Although frequently recovered parental insertions and insertion hotspots reduce the efficiency of gene discovery per plasmid, RescueMu targets a large variety of genes and produces knockout mutants.

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  • Genome Biology, 2004, London: BioMed Central Ltd.

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  • Publication Title: Genome Biology
  • Volume: 5
  • Issue: 10
  • Pages: 20
  • Peer Reviewed: Yes

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UNT Scholarly Works

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  • 2004

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  • March 9, 2012, 2:17 p.m.

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  • April 2, 2014, 2:27 p.m.

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Fernandes, John; Dong, Qunfeng; Schneider, Bret; Morrow, Darren J.; Nan, Guo-Ling; Brendel, Volker et al. Genome-wide mutagenesis of Zea mays L. using RescueMu transposons, article, 2004; [London, United Kingdom]. (digital.library.unt.edu/ark:/67531/metadc77168/: accessed October 18, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT College of Arts and Sciences.