High speed DNA sequence analysis by matrix-assisted laser desorption mass spectrometry. Final report for period February 15, 1991 - February 14, 2001

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Description

This grant had as its focus (i) to develop chemistry and enzymology to permit the enzymatic synthesis of 2' fluoro modified Sanger sequencing reactions, which would be resistant to fragmentation during MALDI process, (ii) to develop rapid MALDI analyses of DNA sequence polymorphisms using Peptide Nucleic Acid (PNA) DNA analogs in conjunction with solid phase chemistry (iii) to study the fundamental mechanisms occurring in the MALDI analysis of nucleic acids.

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5 p.

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Smith, Lloyd M. April 17, 2001.

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Description

This grant had as its focus (i) to develop chemistry and enzymology to permit the enzymatic synthesis of 2' fluoro modified Sanger sequencing reactions, which would be resistant to fragmentation during MALDI process, (ii) to develop rapid MALDI analyses of DNA sequence polymorphisms using Peptide Nucleic Acid (PNA) DNA analogs in conjunction with solid phase chemistry (iii) to study the fundamental mechanisms occurring in the MALDI analysis of nucleic acids.

Physical Description

5 p.

Notes

OSTI as DE00798154

Medium: P; Size: 5 pages

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  • Other Information: PBD: 17 Apr 2001

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  • Report No.: DOE/ER/61130-10
  • Grant Number: FG02-91ER61130
  • DOI: 10.2172/798154 | External Link
  • Office of Scientific & Technical Information Report Number: 798154
  • Archival Resource Key: ark:/67531/metadc742239

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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Creation Date

  • April 17, 2001

Added to The UNT Digital Library

  • Oct. 19, 2015, 7:39 p.m.

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  • April 11, 2017, 12:36 p.m.

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Smith, Lloyd M. High speed DNA sequence analysis by matrix-assisted laser desorption mass spectrometry. Final report for period February 15, 1991 - February 14, 2001, report, April 17, 2001; United States. (digital.library.unt.edu/ark:/67531/metadc742239/: accessed October 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.