Evolutionarily conserved sequences on human chromosome 21

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Comparison of human sequences with the DNA of other mammals is an excellent means of identifying functional elements in the human genome. Here we describe the utility of high-density oligonucleotide arrays as a rapid approach for comparing human sequences with the DNA of multiple species whose sequences are not presently available. High-density arrays representing approximately 22.5 Mb of nonrepetitive human chromosome 21 sequence were synthesized and then hybridized with mouse and dog DNA to identify sequences conserved between humans and mice (human-mouse elements) and between humans and dogs (human-dog elements). Our data show that sequence comparison of multiple species provides ... continued below

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Frazer, Kelly A.; Sheehan, John B.; Stokowski, Renee P.; Chen, Xiyin; Hosseini, Roya; Cheng, Jan-Fang et al. September 1, 2001.

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Comparison of human sequences with the DNA of other mammals is an excellent means of identifying functional elements in the human genome. Here we describe the utility of high-density oligonucleotide arrays as a rapid approach for comparing human sequences with the DNA of multiple species whose sequences are not presently available. High-density arrays representing approximately 22.5 Mb of nonrepetitive human chromosome 21 sequence were synthesized and then hybridized with mouse and dog DNA to identify sequences conserved between humans and mice (human-mouse elements) and between humans and dogs (human-dog elements). Our data show that sequence comparison of multiple species provides a powerful empiric method for identifying actively conserved elements in the human genome. A large fraction of these evolutionarily conserved elements are present in regions on chromosome 21 that do not encode known genes.

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  • Journal Name: Genome Research; Journal Volume: 11; Journal Issue: 10; Other Information: Journal Publication Date: Oct. 2001

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  • Report No.: LBNL--50222
  • Grant Number: AC03-76SF00098
  • DOI: 10.1101/gr.198201 | External Link
  • Office of Scientific & Technical Information Report Number: 795389
  • Archival Resource Key: ark:/67531/metadc739385

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  • September 1, 2001

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  • Oct. 19, 2015, 7:39 p.m.

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  • April 4, 2016, 3:14 p.m.

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Frazer, Kelly A.; Sheehan, John B.; Stokowski, Renee P.; Chen, Xiyin; Hosseini, Roya; Cheng, Jan-Fang et al. Evolutionarily conserved sequences on human chromosome 21, article, September 1, 2001; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc739385/: accessed August 20, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.