DOE final report: Studies on the microbial formation of methane

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The microbial formation of methane is carried out by methanogens which are found wherever active anaerobic degradation of organic matter occurs. We developed a procedure for reliable culture of 'Methanococus jannaschii' which yields 8 g wet weight of cells per liter of medium. To initiate a study of proteomics, this organism was grown at two levels of hydrogen partial pressure, very low (650 Pa) and high (178 kPa). When cells were exposed to hydrogen excess conditions, they possessed very low or undetectable levels of four flagella-related polypeptides, whereas, when hydrogen became limiting, these proteins were synthesized. Thus, use of proteomics ... continued below

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Wolfe, Ralph S. April 1, 2001.

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The microbial formation of methane is carried out by methanogens which are found wherever active anaerobic degradation of organic matter occurs. We developed a procedure for reliable culture of 'Methanococus jannaschii' which yields 8 g wet weight of cells per liter of medium. To initiate a study of proteomics, this organism was grown at two levels of hydrogen partial pressure, very low (650 Pa) and high (178 kPa). When cells were exposed to hydrogen excess conditions, they possessed very low or undetectable levels of four flagella-related polypeptides, whereas, when hydrogen became limiting, these proteins were synthesized. Thus, use of proteomics showed, for the first time, that this methanogen can regulate expression of proteins, and these experiments open the door for general studies of regulation in this hyperthermophile.

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Medium: P; Size: vp.

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OSTI as DE00802852

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  • Other Information: PBD: 1 Apr 2001

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  • Report No.: NONE
  • Grant Number: FG02-87ER13651
  • DOI: 10.2172/802852 | External Link
  • Office of Scientific & Technical Information Report Number: 802852
  • Archival Resource Key: ark:/67531/metadc739198

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  • April 1, 2001

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  • Oct. 19, 2015, 7:39 p.m.

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  • March 21, 2016, 12:38 p.m.

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Wolfe, Ralph S. DOE final report: Studies on the microbial formation of methane, report, April 1, 2001; United States. (digital.library.unt.edu/ark:/67531/metadc739198/: accessed August 21, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.