Extraction of macro-molecule images in cryo-EM micrographs

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Advances in Electron Microscopy and single-particle reconstruction have led to results at increasingly high resolutions. This has opened up the possibility of complete automation of single particle reconstruction. Main bottleneck in automation of single particle reconstruction is manual selection of particles in the micrograph. This paper describes a simple but efficient approach for segmentation of particle projections in the micrographs obtained using cryo-electron microscope. Changing the shape of objects to facilitate segmentation from the cluster and reconstructing its actual shape after isolation is successfully attempted. Both low-level and high-level processing techniques are used and the whole process is made automatic. ... continued below

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Adiga, Umesha P.S.; Malladi, Ravi & Glaeser, Robert M. March 20, 2003.

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Description

Advances in Electron Microscopy and single-particle reconstruction have led to results at increasingly high resolutions. This has opened up the possibility of complete automation of single particle reconstruction. Main bottleneck in automation of single particle reconstruction is manual selection of particles in the micrograph. This paper describes a simple but efficient approach for segmentation of particle projections in the micrographs obtained using cryo-electron microscope. Changing the shape of objects to facilitate segmentation from the cluster and reconstructing its actual shape after isolation is successfully attempted. Both low-level and high-level processing techniques are used and the whole process is made automatic. Over 90 percent success in automatic particle picking is achieved. Several areas for improvement and future research directions are discussed.

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OSTI as DE00810542

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  • Other Information: PBD: 20 Mar 2003

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  • Report No.: LBNL--52387
  • Grant Number: AC03-76SF00098
  • DOI: 10.2172/810542 | External Link
  • Office of Scientific & Technical Information Report Number: 810542
  • Archival Resource Key: ark:/67531/metadc738449

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • March 20, 2003

Added to The UNT Digital Library

  • Oct. 18, 2015, 6:40 p.m.

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  • April 4, 2016, 12:56 p.m.

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Adiga, Umesha P.S.; Malladi, Ravi & Glaeser, Robert M. Extraction of macro-molecule images in cryo-EM micrographs, report, March 20, 2003; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc738449/: accessed November 23, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.