Final progress report, Construction of a genome-wide highly characterized clone resource for genome sequencing

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Description

At TIGR, the human Bacterial Artificial Chromosome (BAC) end sequencing and trimming were with an overall sequencing success rate of 65%. CalTech human BAC libraries A, B, C and D as well as Roswell Park Cancer Institute's library RPCI-11 were used. To date, we have generated >300,000 end sequences from >186,000 human BAC clones with an average read length {approx}460 bp for a total of 141 Mb covering {approx}4.7% of the genome. Over sixty percent of the clones have BAC end sequences (BESs) from both ends representing over five-fold coverage of the genome by the paired-end clones. The average phred ... continued below

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3 p.

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Nierman, William C. February 14, 2000.

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Description

At TIGR, the human Bacterial Artificial Chromosome (BAC) end sequencing and trimming were with an overall sequencing success rate of 65%. CalTech human BAC libraries A, B, C and D as well as Roswell Park Cancer Institute's library RPCI-11 were used. To date, we have generated >300,000 end sequences from >186,000 human BAC clones with an average read length {approx}460 bp for a total of 141 Mb covering {approx}4.7% of the genome. Over sixty percent of the clones have BAC end sequences (BESs) from both ends representing over five-fold coverage of the genome by the paired-end clones. The average phred Q20 length is {approx}400 bp. This high accuracy makes our BESs match the human finished sequences with an average identity of 99% and a match length of 450 bp, and a frequency of one match per 12.8 kb contig sequence. Our sample tracking has ensured a clone tracking accuracy of >90%, which gives researchers a high confidence in (1) retrieving the right clone from the BA C libraries based on the sequence matches; and (2) building a minimum tiling path of sequence-ready clones across the genome and genome assembly scaffolds.

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3 p.

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OSTI as DE00807742

Medium: P; Size: 3 pages

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  • Other Information: PBD: 14 Feb 2000

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  • Report No.: None
  • Grant Number: FC02-97ER62500
  • DOI: 10.2172/807742 | External Link
  • Office of Scientific & Technical Information Report Number: 807742
  • Archival Resource Key: ark:/67531/metadc737567

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  • February 14, 2000

Added to The UNT Digital Library

  • Oct. 18, 2015, 6:40 p.m.

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  • April 10, 2017, 7:22 p.m.

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Nierman, William C. Final progress report, Construction of a genome-wide highly characterized clone resource for genome sequencing, report, February 14, 2000; United States. (digital.library.unt.edu/ark:/67531/metadc737567/: accessed December 18, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.