Miniature Sensors for Biological Warfare Agents using Fatty Acid Profiles: LDRD 10775 Final Report

PDF Version Also Available for Download.

Description

Rapid detection and identification of bacteria and other pathogens is important for many civilian and military applications. The taxonomic significance, or the ability to differentiate one microorganism from another, using fatty acid content and distribution is well known. For analysis fatty acids are usually converted to fatty acid methyl esters (FAMEs). Bench-top methods are commercially available and recent publications have demonstrated that FAMEs can be obtained from whole bacterial cells in an in situ single-step pyrolysis/methylation analysis. This report documents the progress made during a three year Laboratory Directed Research and Development (LDRD) program funded to investigate the use of ... continued below

Physical Description

102 pages

Creation Information

MOWRY, CURTIS D.; MORGAN, CHRISTINE A.; FRYE-MASON, GREGORY C.; THEISEN, LISA; TRUDELL, DANIEL E.; BACA, QUENTIN J. et al. January 1, 2003.

Context

This report is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. More information about this report can be viewed below.

Who

People and organizations associated with either the creation of this report or its content.

Sponsor

Publisher

  • Sandia National Laboratories
    Publisher Info: Sandia National Labs., Albuquerque, NM, and Livermore, CA (United States)
    Place of Publication: Albuquerque, New Mexico

Provided By

UNT Libraries Government Documents Department

Serving as both a federal and a state depository library, the UNT Libraries Government Documents Department maintains millions of items in a variety of formats. The department is a member of the FDLP Content Partnerships Program and an Affiliated Archive of the National Archives.

Contact Us

What

Descriptive information to help identify this report. Follow the links below to find similar items on the Digital Library.

Description

Rapid detection and identification of bacteria and other pathogens is important for many civilian and military applications. The taxonomic significance, or the ability to differentiate one microorganism from another, using fatty acid content and distribution is well known. For analysis fatty acids are usually converted to fatty acid methyl esters (FAMEs). Bench-top methods are commercially available and recent publications have demonstrated that FAMEs can be obtained from whole bacterial cells in an in situ single-step pyrolysis/methylation analysis. This report documents the progress made during a three year Laboratory Directed Research and Development (LDRD) program funded to investigate the use of microfabricated components (developed for other sensing applications) for the rapid identification of bioorganisms based upon pyrolysis and FAME analysis. Components investigated include a micropyrolyzer, a microGC, and a surface acoustic wave (SAW) array detector. Results demonstrate that the micropyrolyzer can pyrolyze whole cell bacteria samples using only milliwatts of power to produce FAMEs from bacterial samples. The microGC is shown to separate FAMEs of biological interest, and the SAW array is shown to detect volatile FAMEs. Results for each component and their capabilities and limitations are presented and discussed. This project has produced the first published work showing successful pyrolysis/methylation of fatty acids and related analytes using a microfabricated pyrolysis device.

Physical Description

102 pages

Source

  • Other Information: PBD: 1 Jan 2003

Language

Item Type

Identifier

Unique identifying numbers for this report in the Digital Library or other systems.

  • Report No.: SAND2003-0168
  • Grant Number: AC04-94AL85000
  • DOI: 10.2172/808624 | External Link
  • Office of Scientific & Technical Information Report Number: 808624
  • Archival Resource Key: ark:/67531/metadc737227

Collections

This report is part of the following collection of related materials.

Office of Scientific & Technical Information Technical Reports

What responsibilities do I have when using this report?

When

Dates and time periods associated with this report.

Creation Date

  • January 1, 2003

Added to The UNT Digital Library

  • Oct. 18, 2015, 6:40 p.m.

Description Last Updated

  • April 11, 2016, 7:14 p.m.

Usage Statistics

When was this report last used?

Yesterday: 0
Past 30 days: 0
Total Uses: 3

Interact With This Report

Here are some suggestions for what to do next.

Start Reading

PDF Version Also Available for Download.

Citations, Rights, Re-Use

MOWRY, CURTIS D.; MORGAN, CHRISTINE A.; FRYE-MASON, GREGORY C.; THEISEN, LISA; TRUDELL, DANIEL E.; BACA, QUENTIN J. et al. Miniature Sensors for Biological Warfare Agents using Fatty Acid Profiles: LDRD 10775 Final Report, report, January 1, 2003; Albuquerque, New Mexico. (digital.library.unt.edu/ark:/67531/metadc737227/: accessed September 25, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.