SIMULTANEOUS MEASUREMENT OF CIRCULAR DICHROISM AND FLUORESCENCE POLARIZATION ANISOTROPY.

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Circular dichroism and fluorescence polarization anisotropy are important tools for characterizing biomolecular systems. Both are used extensively in kinetic experiments involving stopped- or continuous flow systems as well as titrations and steady-state spectroscopy. This paper presents the theory for determining circular dichroism and fluorescence polarization anisotropy simultaneously, thus insuring the two parameters are recorded under exactly the same conditions and at exactly the same time in kinetic experiments. The approach to measuring circular dichroism is that used in almost all conventional dichrographs. Two arrangements for measuring fluorescence polarization anisotropy are described. One uses a single fluorescence detector and signal processing ... continued below

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11 pages

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SUTHERLAND,J.C. January 19, 2002.

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Description

Circular dichroism and fluorescence polarization anisotropy are important tools for characterizing biomolecular systems. Both are used extensively in kinetic experiments involving stopped- or continuous flow systems as well as titrations and steady-state spectroscopy. This paper presents the theory for determining circular dichroism and fluorescence polarization anisotropy simultaneously, thus insuring the two parameters are recorded under exactly the same conditions and at exactly the same time in kinetic experiments. The approach to measuring circular dichroism is that used in almost all conventional dichrographs. Two arrangements for measuring fluorescence polarization anisotropy are described. One uses a single fluorescence detector and signal processing with a lock-in amplifier that is similar to the measurement of circular dichroism. The second approach uses classic ''T'' format detection optics, and thus can be used with conventional photon-counting detection electronics. Simple extensions permit the simultaneous measurement of the absorption and excitation intensity corrected fluorescence intensity.

Physical Description

11 pages

Notes

Refer requests to OSTI; OSTI as DE00791772

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  • PHOTONICS WEST 2002, SAN JOSE, CA (US), 01/19/2002--01/25/2002; Other Information: PROCEEDINGS OF SPIE VOLUME 4633:COMMERCIAL AND BIOMEDICAL APPLICATIONS OF ULTRAFAST AND FREE ELECTRON LASERS, J. NEEV; A. OSTENDORF, EDITORS, THE INTERNATIONAL SOCIETY OF OPTICAL ENGINEERING, BELLINGHAM, WA

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  • Report No.: BNL--68996
  • Grant Number: AC02-98CH10886
  • Office of Scientific & Technical Information Report Number: 791772
  • Archival Resource Key: ark:/67531/metadc734602

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Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • January 19, 2002

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  • Oct. 19, 2015, 7:39 p.m.

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  • Nov. 9, 2015, 8:52 p.m.

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SUTHERLAND,J.C. SIMULTANEOUS MEASUREMENT OF CIRCULAR DICHROISM AND FLUORESCENCE POLARIZATION ANISOTROPY., article, January 19, 2002; Upton, New York. (digital.library.unt.edu/ark:/67531/metadc734602/: accessed December 17, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.