ENHANCED PRACTICAL PHOTOSYNTHETIC CO2 MITIGATION

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This quarterly report documents significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation project during the period from 10/2/2001 through 1/01/2003. As indicated in the list of accomplishments below our current efforts are focused on evaluating candidate organisms and growth surfaces, preparing to conduct long-term tests in the bench-scale bioreactor test systems, and scaling-up the test facilities from bench scale to pilot scale. Specific results and accomplishments for the first quarter of 2003 include: Organisms and Growth Surfaces: (1) Additional thermal features with developed cyanobacterial mats, which might be calcium resistant, were found in the West Thumb area of ... continued below

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17 pages

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Kremer, Dr. Gregory; Bayless, Dr. David J.; Vis, Dr. Morgan; Prudich, Dr. Michael; Cooksey, Dr. Keith & Muhs, Dr. Jeff January 15, 2003.

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Description

This quarterly report documents significant achievements in the Enhanced Practical Photosynthetic CO{sub 2} Mitigation project during the period from 10/2/2001 through 1/01/2003. As indicated in the list of accomplishments below our current efforts are focused on evaluating candidate organisms and growth surfaces, preparing to conduct long-term tests in the bench-scale bioreactor test systems, and scaling-up the test facilities from bench scale to pilot scale. Specific results and accomplishments for the first quarter of 2003 include: Organisms and Growth Surfaces: (1) Additional thermal features with developed cyanobacterial mats, which might be calcium resistant, were found in the West Thumb area of YNP. New samples were isolated and are being cultured in glass tubes. (2) We checked the motile ability of 8.2.1 Synechococcus s.c. (10) and 3.2.2 Synechococcus s.c. 6. It was found that unicellular isolates 8.2.1 Synechococcus s.c. (10) and 3.2.2 Synechococcus s.c. 1 are phototaxic. Isolate 3.2.2 Synechococcus s.c. 1 currently consists of two populations: one population appears to be positive phototaxic, and second population appears negative phototaxis to the same level of light. This means that the character of screen illumination should be uniform and reasonable for cyanobacterial cells. (3) The aeration of growth media with 5% CO{sub 2} in air stimulates cyanobacterial growth 10-20 times over that with air alone. It is possible the rate of the stimulation of cyanobacterial growth in CRF will be higher because cyanobacteria will be grown as a biofilm. We plan to increase the concentration to 15% CO{sub 2} in air. (4) We are continuing the organizing of our collection of the thermophilic cyanobacteria isolated from Yellowstone National Park. During this reporting period we transferred about 160 samples and discarded about 80 samples with weak growth in standard media as BG-11, D or DH. As result of this work we currently have 13 unialgal cultures of thermophilic cyanobacteria. (5) We are screening the cultures to measure the effects of Omnisil on the growth of 2.1 (III) Mastigicladus laminosum, 8.2.1 Synechococcus s.c.10, 1.2 s.c. 6 Chlorogleopsis spp. and 3.3.2 Synechococcus s.c.1. It was found to date that only 1.2 Chlorogleopsis s.c.6 was able to grow in batch culture in the presence of Omnisil. At the moment we have no explanation for the toxic effect of Omnisil, if in fact it is merely spun silica. Nonetheless, we started the selection of Omnisil resistant clones among several cyanobacterial isolates. This process may require several months. Bioreactor support systems and test facilities: (1) A series of tests was run to demonstrate that the initial mass of algae loaded into the CRF-2 system can be accurately determined based on a statistical sampling procedure. Results from the series of tests indicate that the sampling method can be used to reliably estimate the initial algae mass for a CRF-2 test with about 3% uncertainty. (2) Survivability tests for Sc1.2(2) on Omnisil using drilled-hole header inserts are currently underway. Numerous system problems have delayed the testing, but we are hopeful that the debugging of the system is now complete and the current test is proceeding well. Full test results will be provided in the next quarterly report. (3) Initial tests of both ''drilled hole'' and ''pressure shim'' inserts for the integrated wetting/harvesting screens were successful. Both designs showed good flow characteristics and there was no appreciable clogging noticed during and after the test. Test results for flow rate vs. pressure for three header insert designs showed that the performance of the 0.02 inch shim and the drilled hole design are very similar, and thickening the shim to 0.025 inch has the expected effect of reducing the flow at a given pressure for the shim design, but the basic pressure-flow relationship retains the same form. (4) Since the header inserts will likely be manufactured from stainless steel, leaching tests were carried out to find the tolerance of the organisms to SS 316. The organism SC1.2 was very tolerant to SS 316 and it had no effect on the growth of the organism. (5) The pilot-scale bioreactor construction and debugging is continuing on schedule. We are currently waiting for results from the CRF-2 before finalizing the system design.

Physical Description

17 pages

Notes

OSTI as DE00813673

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  • Other Information: PBD: 15 Jan 2003

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  • Report No.: NONE
  • Grant Number: FC26-00NT40932
  • DOI: 10.2172/813673 | External Link
  • Office of Scientific & Technical Information Report Number: 813673
  • Archival Resource Key: ark:/67531/metadc734383

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  • January 15, 2003

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  • Oct. 18, 2015, 6:40 p.m.

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  • Jan. 3, 2017, 12:26 p.m.

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Kremer, Dr. Gregory; Bayless, Dr. David J.; Vis, Dr. Morgan; Prudich, Dr. Michael; Cooksey, Dr. Keith & Muhs, Dr. Jeff. ENHANCED PRACTICAL PHOTOSYNTHETIC CO2 MITIGATION, report, January 15, 2003; United States. (digital.library.unt.edu/ark:/67531/metadc734383/: accessed August 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.