Thermostabilization of desulfurization enzymes from Rhodococcos sp. IGTS8. Final technical report

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The objective of this project was to develop thermophilic cultures capable of expressing the desulfurization (dsz) operon of Rhodococcus sp. IGTS8. The approaches taken in this project included the development of plasmid and integrative expression vectors that function well in Thermus thermophilus, the cloning of Rhodococcus dsz genes in Thermus expression vectors, and the isolation of bacterial cultures that express the dsz operon at thermophilic temperatures. This project has resulted in the development of plasmid and integrative expression vectors for use in T. thermophilus. The dsz genes have been expressed at moderately thermophilic temperatures (52 C) in Mycobacterium phlei and ... continued below

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Medium: P; Size: 73 pages

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II, John J. Kilbane December 15, 2000.

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Description

The objective of this project was to develop thermophilic cultures capable of expressing the desulfurization (dsz) operon of Rhodococcus sp. IGTS8. The approaches taken in this project included the development of plasmid and integrative expression vectors that function well in Thermus thermophilus, the cloning of Rhodococcus dsz genes in Thermus expression vectors, and the isolation of bacterial cultures that express the dsz operon at thermophilic temperatures. This project has resulted in the development of plasmid and integrative expression vectors for use in T. thermophilus. The dsz genes have been expressed at moderately thermophilic temperatures (52 C) in Mycobacterium phlei and at temperatures as high as 72 C in T. thermophilus. The tools and methods developed in this project will be generally useful for the expression of heterologous genes in Thermus. Key developments in the project have been the isolation of a Mycobacterium phlei culture capable of expressing the desulfurization operon at 52 C, development of plasmid and integrative expression vectors for Thermus thermophilus, and the development of a host-vector system based on the malate dehydrogenase gene that allows plasmids to be stably maintained in T. thermophilus and provides a convenient reporter gene for the accurate quantification of gene expression. Publications have been prepared regarding each of these topics; these preprints are included.

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Medium: P; Size: 73 pages

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  • Other Information: PBD: 15 Dec 2000

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  • Report No.: NONE
  • Grant Number: FG02-97ER62464
  • DOI: 10.2172/809375 | External Link
  • Office of Scientific & Technical Information Report Number: 809375
  • Archival Resource Key: ark:/67531/metadc733719

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • December 15, 2000

Added to The UNT Digital Library

  • Oct. 18, 2015, 6:40 p.m.

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  • March 28, 2016, 11:18 p.m.

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II, John J. Kilbane. Thermostabilization of desulfurization enzymes from Rhodococcos sp. IGTS8. Final technical report, report, December 15, 2000; United States. (digital.library.unt.edu/ark:/67531/metadc733719/: accessed December 12, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.