Detecting exposure to environmental organic toxins in individual cells: towards development of a micro-fabricated device

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Description

A new method is being developed to quickly screen for the human exposure potential to polycyclic aromatic hydrocarbons (PAHs) and organochlorines (OCs). The development involves two key elements: identifying suitable signals that represent intracellular changes that are specific to PAH and OC exposure, and constructing a device to guide the biological cell growth so that signals from individual cells are consistent and reproducible. We are completing the identification of suitable signals by using synchrotron radiation-based (SR) Fourier-transform infrared (FTIR) spectromicroscopy in the mid-infrared region (4000-400 cm-1). Distinct changes have been observed in the IR spectra after treatment of human cells ... continued below

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8 pages

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Holman, Hoi-Ying N.; Zhang, Miqin; Goth-Goldstein, Regine; Martin, Michael C.; Russell, Marion; McKinney, Wayne R. et al. January 10, 1999.

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Description

A new method is being developed to quickly screen for the human exposure potential to polycyclic aromatic hydrocarbons (PAHs) and organochlorines (OCs). The development involves two key elements: identifying suitable signals that represent intracellular changes that are specific to PAH and OC exposure, and constructing a device to guide the biological cell growth so that signals from individual cells are consistent and reproducible. We are completing the identification of suitable signals by using synchrotron radiation-based (SR) Fourier-transform infrared (FTIR) spectromicroscopy in the mid-infrared region (4000-400 cm-1). Distinct changes have been observed in the IR spectra after treatment of human cells in culture medium with PAHs and OCs. The potential use of this method for detecting exposure to PAHs and OCs has been tested and compared to a reverse transcription polymerase chain reaction (RT-PCR) assay that quantifies increased expression of the CYP1A1 gene in response to exposu re to PAHs or OCs.

Physical Description

8 pages

Notes

INIS; OSTI as DE00767518

Source

  • SPIE's BiOS '99, San Jose, CA (US), 01/25/1999--01/26/1999

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  • Report No.: LBNL--43749
  • Grant Number: AC03-76SF00098
  • Office of Scientific & Technical Information Report Number: 767518
  • Archival Resource Key: ark:/67531/metadc722234

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  • January 10, 1999

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  • Sept. 29, 2015, 5:31 a.m.

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  • April 4, 2016, 12:38 p.m.

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Holman, Hoi-Ying N.; Zhang, Miqin; Goth-Goldstein, Regine; Martin, Michael C.; Russell, Marion; McKinney, Wayne R. et al. Detecting exposure to environmental organic toxins in individual cells: towards development of a micro-fabricated device, article, January 10, 1999; Berkeley, California. (digital.library.unt.edu/ark:/67531/metadc722234/: accessed August 16, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.