Enumeration of Microbial Populations in Radioactive Environments by Epifluorescence Microscopy

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Epifluorescence microscopy was utilized to enumerate halophilic bacterial populations in two studies involving inoculated, actual radioactive waste/brine mixtures and pure brine solutions. The studies include an initial set of experiments designed to elucidate potential transformations of actinide-containing wastes under salt-repository conditions, including microbially mediated changes. The first study included periodic enumeration of bacterial populations of a mixed inoculum initially added to a collection of test containers. The contents of the test containers are the different types of actual radioactive waste that could potentially be stored in nuclear waste repositories in a salt environment. The transuranic waste was generated from materials ... continued below

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7 p.

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Pansoy-Hjelvik, M.E.A.; Strietelmeierr, B.A.; Paffett, M.T.; Kitten, S.M.; Leonard, P.A.; Dunn, M. et al. December 2, 1996.

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Description

Epifluorescence microscopy was utilized to enumerate halophilic bacterial populations in two studies involving inoculated, actual radioactive waste/brine mixtures and pure brine solutions. The studies include an initial set of experiments designed to elucidate potential transformations of actinide-containing wastes under salt-repository conditions, including microbially mediated changes. The first study included periodic enumeration of bacterial populations of a mixed inoculum initially added to a collection of test containers. The contents of the test containers are the different types of actual radioactive waste that could potentially be stored in nuclear waste repositories in a salt environment. The transuranic waste was generated from materials used in actinide laboratory research. The results show that cell numbers decreased with time. Sorption of the bacteria to solid surfaces in the test system is discussed as a possible mechanism for the decrease in cell numbers. The second study was designed to determine radiological and/or chemical effects of {sup 239}Pu, {sup 243}Am, {sup 237}Np, {sup 232}Th and {sup 238}U on the growth of pure and mixed anaerobic, denitrifying bacterial cultures in brine media. Pu, Am, and Np isotopes at concentrations of <=1 x 10{sup {minus}5}M, <=5 x 10{sup {minus}6}M and <=5 x 10{sup {minus}4}M respectively, and Th and U isotopes <=4 x 10{sup {minus}3}M were tested in these media. The results indicate that high actinide concentration affected both the bacterial growth rate and morphology. However, relatively minor effects from Am were observed at all tested concentrations with the pure culture.

Physical Description

7 p.

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INIS; OSTI as DE00760108

Medium: P; Size: 7 pages

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  • Material Research Society Meeting, Boston, MA (US), 12/02/1996--12/06/1996

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  • Report No.: LA-UR-97-253
  • Grant Number: W-7405-ENG-36
  • Office of Scientific & Technical Information Report Number: 760108
  • Archival Resource Key: ark:/67531/metadc720177

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  • December 2, 1996

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  • Sept. 29, 2015, 5:31 a.m.

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  • April 11, 2017, 3:15 p.m.

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Pansoy-Hjelvik, M.E.A.; Strietelmeierr, B.A.; Paffett, M.T.; Kitten, S.M.; Leonard, P.A.; Dunn, M. et al. Enumeration of Microbial Populations in Radioactive Environments by Epifluorescence Microscopy, article, December 2, 1996; New Mexico. (digital.library.unt.edu/ark:/67531/metadc720177/: accessed January 16, 2019), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.