Large microchannel array fabrication and results for DNA sequencing

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We have developed a process for the production of microchannel arrays on bonded glass substrates up to I4 x 58 cm, for DNA sequencing. Arrays of 96 and 384 microchannels, each 46 cm long have been built. This technology offers significant advantages over discrete capillaries or conventional slab-gel approaches. High throughput DNA sequencing with over 550 base pairs resolution has been achieved. With custom fabrication apparatus, microchannels are etched in a borosilicate substrate, and then fusion bonded to a top substrate 1.1 mm thick that has access holes formed in it. SEM examination shows a typical microchannel to be 40 ... continued below

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Pastrone, R L; Balch, J W; Brewer, L R; Copeland, A C; Davidson , J C; Fitch, J P et al. January 7, 1999.

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We have developed a process for the production of microchannel arrays on bonded glass substrates up to I4 x 58 cm, for DNA sequencing. Arrays of 96 and 384 microchannels, each 46 cm long have been built. This technology offers significant advantages over discrete capillaries or conventional slab-gel approaches. High throughput DNA sequencing with over 550 base pairs resolution has been achieved. With custom fabrication apparatus, microchannels are etched in a borosilicate substrate, and then fusion bonded to a top substrate 1.1 mm thick that has access holes formed in it. SEM examination shows a typical microchannel to be 40 x 180 micrometers by 46 cm Iong; the etch is approximately isotropic, leaving a key undercut, for forming a rounded channel. The surface roughness at the bottom of the 40 micrometer deep channel has been profilometer measured to be as low as 20 nm; the roughness at the top surface was 2 nm. Etch uniformity of about 5% has been obtained using a 22% vol. HF / 78% Acetic acid solution. The simple lithography, etching, and bonding of these substrates enables efficient production of these arrays and extremely precise replication From master masks and precision machining with a mandrel. Keywords: microchannels, microchannel plates, DNA sequencing, electrophoresis, borosilicate glass

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2.9 Megabytes

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  • Micro and Nanofabricated Structures and Devices for Biomedical Environmental Applications II, SPIE's BiOS'99 International Biomedical Optics Symposium, San Jose, CA, January 23-29, 1999

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  • Other: DE00007804
  • Report No.: UCRL-JC-132763
  • Grant Number: W-7405-Eng-48
  • Office of Scientific & Technical Information Report Number: 7804
  • Archival Resource Key: ark:/67531/metadc719765

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  • January 7, 1999

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  • Sept. 29, 2015, 5:31 a.m.

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  • Aug. 8, 2016, 7:39 p.m.

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Pastrone, R L; Balch, J W; Brewer, L R; Copeland, A C; Davidson , J C; Fitch, J P et al. Large microchannel array fabrication and results for DNA sequencing, article, January 7, 1999; Livermore, California. (digital.library.unt.edu/ark:/67531/metadc719765/: accessed August 18, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.