Towards the Development of a Molecular Map in Switchgrass: I. Microsatellite Marker Development

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The long-term goal of the switchgrass breeding program is to improve regionally adapted varieties and increase biomass yield and feedstock quality. Although, to some extent, biomass yields are dependent on environmental constraints, increased yield can be achieved through the development of genotypes with improved seasonal adaptation, tolerance to unfavorable environmental conditions, and improved resistance to pest and disease. To date, improvement in switchgrass has relied on recurrent breeding strategies based on phenotypic or genotypic selection. Yield improvements have been modest by this method. If we expect to make significant increase in yields, we need tools that will allow us to ... continued below

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14 pages

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Gunter, L.E. August 23, 2001.

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Description

The long-term goal of the switchgrass breeding program is to improve regionally adapted varieties and increase biomass yield and feedstock quality. Although, to some extent, biomass yields are dependent on environmental constraints, increased yield can be achieved through the development of genotypes with improved seasonal adaptation, tolerance to unfavorable environmental conditions, and improved resistance to pest and disease. To date, improvement in switchgrass has relied on recurrent breeding strategies based on phenotypic or genotypic selection. Yield improvements have been modest by this method. If we expect to make significant increase in yields, we need tools that will allow us to map complex traits and uncover the genes that influence them. A genetic linkage map could be a powerful tool for accelerating switchgrass development through marker-assisted selection, breeding and recombination. This type of mapping requires the development of markers that can be associated with phenotypic traits in a population of known pedigree. The most commonly used markers for mapping include restriction fragment length polymorphisms (RFLP) and simple sequence repeats (SSR). At ORNL, we have been concentrating on the development of SSR markers, while our colleagues at the University of Georgia are developing RFLP markers in order to select parents to produce a mapping population and from there to create a framework map from {approx}100 F1 progeny.

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14 pages

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  • Other Information: PBD: 23 Aug 2001

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  • Report No.: R01-111162
  • Grant Number: AC05-00OR22725
  • DOI: 10.2172/788504 | External Link
  • Office of Scientific & Technical Information Report Number: 788504
  • Archival Resource Key: ark:/67531/metadc718679

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  • August 23, 2001

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  • Sept. 29, 2015, 5:31 a.m.

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  • March 30, 2016, 4:47 p.m.

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Gunter, L.E. Towards the Development of a Molecular Map in Switchgrass: I. Microsatellite Marker Development, report, August 23, 2001; Tennessee. (digital.library.unt.edu/ark:/67531/metadc718679/: accessed September 25, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.