Degradation of hazardous chemicals in liquid radioactive wastes from biomedical research using a mixed microbial population

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Description

As the costs associated with treatment of mixed wastes by conventional methods increase, new technologies will be investigated as alternatives. This study examines the potential of using a selected mixed population of microorganisms to treat hazardous chemical compounds in liquid low level radioactive wastes from biomedical research procedures. Microorganisms were isolated from various waste samples and enriched against compounds known to occur in the wastes. Individual isolates were tested for their ability to degrade methanol, ethanol, phenol, toluene, phthalates, acetonitrile, chloroform, and trichloroacetic acid. Following these tests, the organisms were combined in a media with a mixture of the different ... continued below

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29 p.

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Wolfram, J.H.; Radtke, M.; Wey, J.E.; Rogers, R.D. & Rau, E.H. October 1, 1997.

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Description

As the costs associated with treatment of mixed wastes by conventional methods increase, new technologies will be investigated as alternatives. This study examines the potential of using a selected mixed population of microorganisms to treat hazardous chemical compounds in liquid low level radioactive wastes from biomedical research procedures. Microorganisms were isolated from various waste samples and enriched against compounds known to occur in the wastes. Individual isolates were tested for their ability to degrade methanol, ethanol, phenol, toluene, phthalates, acetonitrile, chloroform, and trichloroacetic acid. Following these tests, the organisms were combined in a media with a mixture of the different compounds. Three compounds: methanol, acetonitrile, and pseudocumene, were combined at 500 microliter/liter each. Degradation of each compound was shown to occur (75% or greater) under batch conditions with the mixed population. Actual wastes were tested by adding an aliquot to the media, determining the biomass increase, and monitoring the disappearance of the compounds. The compounds in actual waste were degraded, but at different rates than the batch cultures that did not have waste added. The potential of using bioprocessing methods for treating mixed wastes from biomedical research is discussed.

Physical Description

29 p.

Notes

INIS; OSTI as DE98050320

Source

  • 4. biennial ASME mixed waste symposium, Baltimore, MD (United States), 17-21 Aug 1997

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  • Other: DE98050320
  • Report No.: INEEL/CON--97-00119
  • Report No.: CONF-970857--
  • Grant Number: AC07-94ID13223
  • DOI: 10.2172/671847 | External Link
  • Office of Scientific & Technical Information Report Number: 671847
  • Archival Resource Key: ark:/67531/metadc711228

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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Creation Date

  • October 1, 1997

Added to The UNT Digital Library

  • Sept. 12, 2015, 6:31 a.m.

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  • June 14, 2016, 2:27 p.m.

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Wolfram, J.H.; Radtke, M.; Wey, J.E.; Rogers, R.D. & Rau, E.H. Degradation of hazardous chemicals in liquid radioactive wastes from biomedical research using a mixed microbial population, report, October 1, 1997; Idaho Falls, Idaho. (digital.library.unt.edu/ark:/67531/metadc711228/: accessed May 20, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.