High-resolution x-ray imaging for microbiology at the Advanced Photon Source

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Exciting new applications of high-resolution x-ray imaging have emerged recently due to major advances in high-brilliance synchrotrons sources and high-performance zone plate optics. Imaging with submicron resolution is now routine with hard x-rays: the authors have demonstrated 150 run in the 6--10 keV range with x-ray microscopes at the Advanced Photon Source (APS), a third-generation synchrotrons radiation facility. This has fueled interest in using x-ray imaging in applications ranging from the biomedical, environmental, and materials science fields to the microelectronics industry. One important application they have pursued at the APS is a study of the microbiology of bacteria and their ... continued below

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7 p.

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Lai, B.; Kemner, K. M.; Maser, J.; Schneegurt, M. A.; Cai, Z.; Ilinski, P. P. et al. November 2, 1999.

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Description

Exciting new applications of high-resolution x-ray imaging have emerged recently due to major advances in high-brilliance synchrotrons sources and high-performance zone plate optics. Imaging with submicron resolution is now routine with hard x-rays: the authors have demonstrated 150 run in the 6--10 keV range with x-ray microscopes at the Advanced Photon Source (APS), a third-generation synchrotrons radiation facility. This has fueled interest in using x-ray imaging in applications ranging from the biomedical, environmental, and materials science fields to the microelectronics industry. One important application they have pursued at the APS is a study of the microbiology of bacteria and their associated extracellular material (biofilms) using fluorescence microanalysis. No microscopy techniques were previously available with sufficient resolution to study live bacteria ({approx}1 {micro}m x 4 {micro}m in size) and biofilms in their natural hydrated state with better than part-per-million elemental sensitivity and the capability of determining g chemical speciation. In vivo x-ray imaging minimizes artifacts due to sample fixation, drying, and staining. This provides key insights into the transport of metal contaminants by bacteria in the environment and potential new designs for remediation and sequestration strategies.

Physical Description

7 p.

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INIS; OSTI as DE00750571

Medium: P; Size: 7 pages

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  • X-ray and Inner Shell Processes: 18th International Conference, Argonne, IL (US), 08/23/1999--08/27/1999

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  • Report No.: ANL/XFD/CP-100369
  • Grant Number: W-31109-ENG-38
  • Office of Scientific & Technical Information Report Number: 750571
  • Archival Resource Key: ark:/67531/metadc710844

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

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  • November 2, 1999

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  • Sept. 12, 2015, 6:31 a.m.

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  • April 11, 2017, 3:27 p.m.

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Lai, B.; Kemner, K. M.; Maser, J.; Schneegurt, M. A.; Cai, Z.; Ilinski, P. P. et al. High-resolution x-ray imaging for microbiology at the Advanced Photon Source, article, November 2, 1999; Illinois. (digital.library.unt.edu/ark:/67531/metadc710844/: accessed October 23, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.