The effect of pretreatments on surfactin production from potato process effluent by Bacillus subtilis

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Pretreatment of low-solids (LS) potato process effluent was tested for potential to increase surfactin yield. Pretreatments included heat, removal of starch particulates, and acid hydrolysis. Elimination of contaminating vegetative cells was necessary for surfactin production. After autoclaving, 0.40 g/L of surfactin was produced from the effluent in 72 h, versus 0.24 g/L in the purified potato starch control. However, surfactin yields per carbon consumed were 76% lower from process effluent. Removal of starch particulates had little effect on the culture. Acid hydrolysis decreased growth and surfactant production, except 0.5 wt% acid, which increased the yield by 25% over untreated effluent.

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129 Kilobytes pages

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Thompson, D. N.; Fox, S. L. & Bala, G. A. May 7, 2000.

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Pretreatment of low-solids (LS) potato process effluent was tested for potential to increase surfactin yield. Pretreatments included heat, removal of starch particulates, and acid hydrolysis. Elimination of contaminating vegetative cells was necessary for surfactin production. After autoclaving, 0.40 g/L of surfactin was produced from the effluent in 72 h, versus 0.24 g/L in the purified potato starch control. However, surfactin yields per carbon consumed were 76% lower from process effluent. Removal of starch particulates had little effect on the culture. Acid hydrolysis decreased growth and surfactant production, except 0.5 wt% acid, which increased the yield by 25% over untreated effluent.

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129 Kilobytes pages

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OSTI as DE00758109

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  • 22nd Symposium on Biotechnology for Fuels and Chemicals, Gatlinburg, TN (US), 05/07/2000--05/11/2000

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  • Report No.: INEEL/CON-99-01278
  • Grant Number: AC07-99ID13727
  • Office of Scientific & Technical Information Report Number: 758109
  • Archival Resource Key: ark:/67531/metadc710510

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  • May 7, 2000

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  • Sept. 12, 2015, 6:31 a.m.

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  • April 27, 2016, 3:34 p.m.

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Thompson, D. N.; Fox, S. L. & Bala, G. A. The effect of pretreatments on surfactin production from potato process effluent by Bacillus subtilis, article, May 7, 2000; Idaho Falls, Idaho. (digital.library.unt.edu/ark:/67531/metadc710510/: accessed August 19, 2017), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.