Survival and activity of Streptococcus faecalis and Escherichia coli in tropical freshwater

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The survival of Streptococcus facecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. facecalis and E. coli decreased less than 1 log unit after 105 h as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 h, E. coli was more active than S. faecalis ... continued below

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24 p.

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Muniz, I.; Jimenez, L.; Toranzos, G. A. & Hazen, T. C. Spring 1988.

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This report is part of the collection entitled: Office of Scientific & Technical Information Technical Reports and was provided by UNT Libraries Government Documents Department to Digital Library, a digital repository hosted by the UNT Libraries. It has been viewed 155 times . More information about this report can be viewed below.

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  • Savannah River Laboratory
    Publisher Info: E.I. DuPont de Nemours and Co., Savannah River Lab., Aiken, SC (United States)
    Place of Publication: Aiken, South Carolina

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Description

The survival of Streptococcus facecalis and Escherichia coli was studied in situ in a tropical rain forest watershed using membrane diffusion chambers. Densities were determined by acridine orange direct count and Coulter Counter. Population activity was determined by microautoradiography, cell respiration, and by nucleic acid composition. Densities of S. facecalis and E. coli decreased less than 1 log unit after 105 h as measured by direct count methods. Activity as measured by respiration, acridine orange activity, and microautoradiography indicated that both bacteria remained moderately active during the entire study. After 12 h, E. coli was more active than S. faecalis as measured by nucleic acid composition. E. coli and S. faecalis survived and remained active for more than 5 days. Consequently, both would seem to be unsuitable as indicators of recent fecal contamination in tropical waters.

Physical Description

24 p.

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OSTI as DE98007423

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  • Other Information: PBD: [1988]

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  • Other: DE98007423
  • Report No.: DP-MS--88-202
  • Grant Number: AC09-89SR18035;AC09-76SR00001
  • DOI: 10.2172/666262 | External Link
  • Office of Scientific & Technical Information Report Number: 666262
  • Archival Resource Key: ark:/67531/metadc710291

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  • Spring 1988

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  • Sept. 12, 2015, 6:31 a.m.

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  • March 23, 2016, 11:21 a.m.

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Muniz, I.; Jimenez, L.; Toranzos, G. A. & Hazen, T. C. Survival and activity of Streptococcus faecalis and Escherichia coli in tropical freshwater, report, Spring 1988; Aiken, South Carolina. (digital.library.unt.edu/ark:/67531/metadc710291/: accessed September 18, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.