Ethanol production from dry-mill corn starch in a fluidized-bed bioreactor

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The development of a high-rate process for the production of fuel ethanol from dry-mill corn starch using fluidized-bed bioreactor (FBR) technology is discussed. Experiments were conducted in a laboratory scale FBR using immobilized biocatalysts. Two ethanol production process designs were considered in this study. In the first design, simultaneous saccharification and fermentation was performed at 35 C using {kappa}-carageenan beads (1.5 mm to 1.5 mm in diameter) of co-immobilized glucoamylase and Zymomonas mobilis. For dextrin feed concentration of 100 g/L, the single-pass conversion ranged from 54% to 89%. Ethanol concentrations of 23 to 36 g/L were obtained at volumetric productivities ... continued below

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9 p.

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Krishnan, M. S.; Nghiem, N. P. & Davison, B. H. August 1, 1998.

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The development of a high-rate process for the production of fuel ethanol from dry-mill corn starch using fluidized-bed bioreactor (FBR) technology is discussed. Experiments were conducted in a laboratory scale FBR using immobilized biocatalysts. Two ethanol production process designs were considered in this study. In the first design, simultaneous saccharification and fermentation was performed at 35 C using {kappa}-carageenan beads (1.5 mm to 1.5 mm in diameter) of co-immobilized glucoamylase and Zymomonas mobilis. For dextrin feed concentration of 100 g/L, the single-pass conversion ranged from 54% to 89%. Ethanol concentrations of 23 to 36 g/L were obtained at volumetric productivities of 9 to 15 g/L-h. No accumulation of glucose was observed, indicating that saccharification was the rate-limiting step. In the second design, saccharification and fermentation were carried out sequentially. In the first stage, solutions of 150 to 160 g/L dextrins were pumped through an immobilized glucoamylase packed column maintained at 55 C. Greater than 95% conversion was obtained at a residence time of 1 h, giving a product of 165 to 170 g glucose/L. In the second stage, these glucose solutions were fed to the FBR containing Z. mobilis immobilized in {kappa}-carageenan beads. At a residence time of 2 h, 94% conversion and ethanol concentration of 70 g/L was achieved, giving an overall productivity of 23 g/L-h.

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9 p.

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OSTI as DE98007176

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  • BioEnergy `98 conference, Madison, WI (United States), 4-9 Oct 1998

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  • Other: DE98007176
  • Report No.: ORNL/CP--98828
  • Report No.: CONF-981015--
  • Grant Number: AC05-96OR22464
  • Office of Scientific & Technical Information Report Number: 665978
  • Archival Resource Key: ark:/67531/metadc709634

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  • August 1, 1998

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  • Sept. 12, 2015, 6:31 a.m.

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  • Nov. 3, 2016, 6:27 p.m.

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Krishnan, M. S.; Nghiem, N. P. & Davison, B. H. Ethanol production from dry-mill corn starch in a fluidized-bed bioreactor, article, August 1, 1998; Tennessee. (digital.library.unt.edu/ark:/67531/metadc709634/: accessed August 17, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.