A MEMS-based miniature DNA analysis system

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Description

We detail the design and development of a miniature thermal cycling instrument for performing the polymerase chain reaction (PCR) that uses microfabricated, silicon-based reaction chambers. The MEMS-based, battery-operated instrument shows significant improvements over commercial thermal cycling instrumentation. Several different biological systems have been amplified and verified with the miniature PCR instrument including the Human Immunodeficiency Virus; both cloned and genomic DNA templates of {beta} globin; and the genetic disease, Cystic Fibrosis from human DNA. The miniaturization of a PCR thermal cycler is the initial module of a fully-integrated portable, low-power, rapid, and highly efficient bioanalytical instrument.

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6 p.

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Northrup, M.A.; Gonzalez, C. & Hadley, D. April 25, 1995.

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Description

We detail the design and development of a miniature thermal cycling instrument for performing the polymerase chain reaction (PCR) that uses microfabricated, silicon-based reaction chambers. The MEMS-based, battery-operated instrument shows significant improvements over commercial thermal cycling instrumentation. Several different biological systems have been amplified and verified with the miniature PCR instrument including the Human Immunodeficiency Virus; both cloned and genomic DNA templates of {beta} globin; and the genetic disease, Cystic Fibrosis from human DNA. The miniaturization of a PCR thermal cycler is the initial module of a fully-integrated portable, low-power, rapid, and highly efficient bioanalytical instrument.

Physical Description

6 p.

Notes

OSTI as DE95013266

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  • Transducers `95, Stockholm (Sweden), 25-29 Jun 1995

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  • Other: DE95013266
  • Report No.: UCRL-JC--120812
  • Report No.: CONF-9506192--1
  • Grant Number: W-7405-ENG-48
  • Office of Scientific & Technical Information Report Number: 71671
  • Archival Resource Key: ark:/67531/metadc708109

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Office of Scientific & Technical Information Technical Reports

Reports, articles and other documents harvested from the Office of Scientific and Technical Information.

Office of Scientific and Technical Information (OSTI) is the Department of Energy (DOE) office that collects, preserves, and disseminates DOE-sponsored research and development (R&D) results that are the outcomes of R&D projects or other funded activities at DOE labs and facilities nationwide and grantees at universities and other institutions.

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  • April 25, 1995

Added to The UNT Digital Library

  • Sept. 12, 2015, 6:31 a.m.

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  • Feb. 23, 2016, 1:55 p.m.

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Northrup, M.A.; Gonzalez, C. & Hadley, D. A MEMS-based miniature DNA analysis system, article, April 25, 1995; California. (digital.library.unt.edu/ark:/67531/metadc708109/: accessed July 22, 2018), University of North Texas Libraries, Digital Library, digital.library.unt.edu; crediting UNT Libraries Government Documents Department.